长白山林区3种基原细辛差异性成分比较  

Comparative Analysis of Differential Components of Three Origins of Asari Radix et Rhizoma in Changbai Mountain Forest Region

作　　者：于芸泽 于营[1,2] 赵书文 金银萍 逄世峰[1,2] 张浩 邢丽伟[4] 孙语杨 郭靖[1,2] YU Yunze;YU Ying;ZHAO Shuwen;JIN Yinping;PANG Shifeng;ZHANG Hao;XING Liwei;SUN Yuyang;GUO Jing(Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences,Changchun 130112;Jilin Provincial Key Lab-oratory of Traditional Chinese Medicinal Materials Cultivation and Propagation,Changchun 130112;College of Landscape Architecture,North-east Forestry University,Harbin 150040;Hailar District Agricultural and Animal Husbandry Technology Extension Center,Hulunbuir 021000;Kings College London,London WC2R 2LS)

机构地区：[1]中国农业科学院特产研究所,长春130112 [2]吉林省中药材种植(养殖)重点实验室,长春130112 [3]东北林业大学园林学院,哈尔滨150040 [4]呼伦贝尔市海拉尔区农牧技术推广中心,呼伦贝尔021000 [5]伦敦国王学院,英国伦敦WC2R 2LS

出　　处：《植物研究》2025年第1期66-76,共11页Bulletin of Botanical Research

基　　金：吉林省科技发展计划项目(20230401047YY);中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-ISAPS)。

摘　　要：细辛挥发油中含有多种活性成分,可以作为细辛药材质量评价的指标;理清不同基原细辛挥发油成分差异,对细辛品质研究及应用具有重要意义。利用气质联用(GC-MS)法对不同基原细辛挥发油成分进行定性及相对定量分析,结合数据分析软件,通过聚类分析(HCA)、主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)找出不同基原细辛的差异性化合物。结果表明:25个批次的细辛样品共检测出59种化合物,按照不同基原划分为3类;细辛挥发性成分差异与细辛基原具有明显关联,确定α-松油醇、肉豆蔻醚、莰烯等11种化合物是不同基原细辛的差异性成分。基于化学计量学方法筛选到的差异成分可以区分不同基原细辛,同时可为细辛资源合理利用提供科学依据。The volatile oil contains many kinds of active ingredients,which can be used as an indicator for the quality evaluation of Asari Radix et Rhizoma,and the difference of volatile oil components of different origins is of great significance for the quality research and development and application of Asari Radix et Rhizoma.Gas chromatography-mass spectrometry(GC-MS)was used to qualitatively and relatively quantify the volatile oil components of different origins,combined with data analysis software,and the differential compounds of different origins were identified by cluster analysis(HCA),principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA).The results showed that a total of 59 compounds were detected in 25 batches of Asari Radix et Rhizoma samples,which could be divided into three categories according to different origins.Further analysis showed that the difference in volatile components of Asari Radix et Rhizoma was significantly correlated with different origins,and 11 compounds such asα-terpineol,myristic ether and camphene were identified as different components of different origins.The differential components screened by chemometrics can be used to distinguish different origins and provide a comprehensive reference for the rational use of Asari Radix et Rhizoma resources.

关 键 词：细辛 挥发油 化学计量学 气相色谱质谱 成分分析 

分 类 号：S131[农业科学—农业基础科学] S132[生物学—植物学] Q94.334