吉林省珲春市刺扰伊蚊中辽宁病毒的检测与分析  

Detection and analysis of Liaoning virus from Aedes vexans collected in Hunchun city,Jilin Province

作　　者：阳玉红 金光俊 张帮帅 苟伟民 殷启凯 聂凯[2] 付士红[2] 崔倩倩 许松涛[2] 李樊 李兴洲[1] 王环宇[2] Yang Yuhong;Jin Guangjun;Zhang Bangshuai;Gou Weimin;Yin Qikai;Nie Kai;Fu Shihong;Cui Qianqian;Xu Songtao;Li Fan;Li Xingzhou;Wang Huanyu(School of Public Health,Jiamusi University,Jiamusi 154007,China;National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,NHC Key Laboratory of Biosafety,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Hunchun Center for Disease Control and Prevention,Hunchun 133399,China;Yanbian Center for Disease Control and Prevention,Yanbian 133001,China)

机构地区：[1]佳木斯大学公共卫生学院,佳木斯154007 [2]中国疾病预防控制中心病毒病预防控制所,传染病溯源预警与智能决策全国重点实验室,卫生健康委生物安全重点实验室,北京102206 [3]珲春市疾病预防控制中心,珲春133399 [4]延边州疾病预防控制中心,延边133001

出　　处：《中华实验和临床病毒学杂志》2024年第6期621-627,共7页Chinese Journal of Experimental and Clinical Virology

基　　金：北京市自然科学基金(L232069);国家重点研发计划资助(2022YFC2302700);中国疾病预防控制中心青年基金(2023A103)。

摘　　要：目的对2023年在吉林省珲春市采集的蚊虫标本进行蚊媒病毒的核酸筛查及病毒进化特征分析。方法首先采用形态学方法对蚊虫标本进行分类鉴定,通过实时荧光定量逆转录聚合酶链式反应(qRT-PCR)方法对采集的蚊虫样本进行6种蚊媒病毒核酸筛查,包括版纳病毒(Banna virus,BAV)、卡皮罗病毒(Kadipiro virus,KDV)、辽宁病毒(Liaoning virus,LNV)、塔希纳病毒(Tahyna virus,TAHV)、盖塔病毒(Getah virus,GETV)和乙型脑炎病毒(Japanese encephalitis virus,JEV)。通过测序获取阳性样本的病毒基因组序列。结果本次调查共采集5490只蚊虫标本,包含刺扰伊蚊4400只(80.15%),中华按蚊1090只(19.85%),按采集的时间、地点、蚊种进行混样共获得41组样本。qRT-PCR检测显示,仅编号为JLHC2321的刺扰伊蚊样本呈LNV核酸阳性,其余样本对所检测病毒均呈阴性。针对LNV第10节段基因的系统发育分析显示,该株辽宁病毒与NE9731毒株亲缘关系最接近,属于II型分支。同时基于第10节段编码区(Coding sequence,CDS)的氨基酸序列分析显示,JLHC2321与II型参考株NE9731仅存在2个氨基酸差异位点。结论本研究首次在吉林省珲春市的刺扰伊蚊中检测出LNV,为该区域蚊媒病毒的监测和防控策略提供了重要的基础数据。Objective This study conducted mosquito-borne viruses RNA screening and analysis of virus evolution characteristics on mosquito specimens collected in 2023 from Hunchun city,Jilin province,China.Methods Firstly,morphological method were employed for mosquito specimen classification.Then,real-time fluorescence quantitative reverse transcription-polymerase chain reaction(qRT-PCR)was used to detect the RNA of six mosquito-borne viruses in the collected mosquitos,i.e.,Banna virus(BAV),Kadipiro virus(KDV),Liaoning virus(LNV),Tahyna virus(TAHV),Getah virus(GETV)and Japanese encephalitis virus(JEV).And by sequencing,the viral genome sequence of positive samples was obtained.Results A total of 5490 mosquito specimens were collected from Hunchun city,Jilin province,included 4400 Aedes vexans(80.15%),1090 Anopheles sinensis(19.85%).A total of 41 groups were obtained by mixing samples according to the time,location,and mosquito species collected.qRT-PCR result showed that only the Aedes vexans sample with the number JLHC2321 was tested positive for LNV,while the remaining samples were tested negative for the detected viruses.According to the phylogenetic analysis of the segment 10 gene,this LNV strain had the closest genetic relationship with NE9731 and belonged to the type II branch.Meanwhile,the amino acid sequence analysis based on the coding sequence(CDS)in the segment 10 showed that JLHC2321 only had 2 amino acid differential sites with the GII reference strain NE9731.Conclusions This study detected LNV for the first time in Aedes vexans in Hunchun city,and our result provide important basic data for the monitoring and prevention strategies of mosquito-borne viruses in the region.

关 键 词：蚊媒病毒 辽宁病毒 刺扰伊蚊 

分 类 号：S85[农业科学—兽医学]