云南省阿卡斑病毒和西藏环状病毒全基因组特征研究  

Genomic characterization of Akabane virus and Tibet orbivirus in Yunnan province

作　　者：蒋蓉 韩茜 杨卫红[2,3] 邝国鹏[2,3] 王娟 杨丽芬 潘虹 冯云 Jiang Rong;Han Xi;Yang Weihong;Kuang Guopeng;Wang Juan;Yang Lifen;Pan Hong;Feng Yun(School of Public Health,Dali University,Dali 671000,China;Yunnan Provincial Institute for Endemic Disease Control and Prevention,Dali 671000,China;Key Laboratory of Natural Focal Disease Prevention and Control Technology in Yunnan Province,Dali 671000,China)

机构地区：[1]大理大学公共卫生学院,大理671000 [2]云南省地方病防治所,大理671000 [3]云南省自然疫源性疾病防控技术重点实验室,大理671000

出　　处：《中华实验和临床病毒学杂志》2024年第6期661-668,共8页Chinese Journal of Experimental and Clinical Virology

基　　金：云南兴滇英才支持计划名医项目(XDYC-MY-2022-0074)。

摘　　要：目的阐明2010年从云南省德宏州采集的蚊虫标本分离物中的阿卡斑病毒(Akabane virus,AKV)DHL10M117和西藏环状病毒(Tibet orbivirus,TIBOV)DH10M1019两株毒株的全基因组特征。方法使用宏转录组方法与高通量测序方法获取两种RNA病毒的全基因序列。采用Megalign软件(DNAStar)等生物信息软件进行基因组特征分析。结果获得DHL10M117株S、M、L三个基因节段长度分别为856 bp、4309 bp、6869 bp的全基因组序列;构建系统进化树显示:DHL10M117株为AKV,与AKV云南分离株(DHL10M110)进化关系最近。S和M节段基因进化树中,与我国南方地区以及日本等地区的AKV流行株亲缘关系较近,与澳大利亚等分离株亲缘关系最远,表明该分离株属于亚洲株且地域特征明显;经同源性分析,DHL10M117株S、M、L节段的基因序列均与DHL10M110株的核苷酸(100%)和氨基酸(100%)同源性最高。DH10M1019株Seg-1~Seg-10十个节段基因长度分别为3950 bp、2904 bp、2769 bp、1978 bp、1772 bp、1638 bp、1165 bp、1142 bp、1103 bp、832 bp的全基因组序列;构建系统进化树显示:DH10M1019株为TIBOV,DH10M1019株八个节段基因(Seg-1~Seg-4、Seg-6、Seg-8~Seg-10)都与已知毒株聚集在同一大分支上,但Seg-5、Seg-7两个节段基因则独立于已知参考株自成一个小分支,研究发现DH10M1019株可能为一种新血清型的TIBOV。结论通过全基因组序列分析,证实DHL10M117株和DH10M1019株分别是AKV和TIBOV,为两种病毒的流行特征、致病性分析与调查研究提供科学依据。Objective To elucidate the complete genomic characteristics of the Akabane virus(AKV)DHL10M117 strain and the Tibet orbivirus(TIBOV)DH10M1019 strain,isolated from mosquito specimens collected in 2010 from Dehong prefecture,Yunnan province.Methods The complete RNA virus sequences were obtained using metatranscriptomics and high-throughput sequencing.Results The complete genome sequences of the DHL10M117 strain,consisting of the S,M,and L gene segments with lengths of 856 bp,4309 bp,and 6869 bp,respectively,were acquired.Phylogenetic analysis indicated that the DHL10M117 strain is an AKV strain,closely related to the AKV strain DHL10M110,isolated from Yunnan.The S and M segment phylogenetic trees revealed that the strain is closely related to AKV strains circulating in southern China and Japan,and distantly related to strains from Australia,suggesting that this strain belongs to the Asian lineage with distinct regional characteristics.Homology analysis confirmed that the gene sequences of the S,M,and L segments of the DHL10M117 strain showed 100%nucleotide and amino acid identity with the DHL10M110 strain.Additionally,the complete genome sequences of the DH10M1019 strain,comprising ten gene segments(Seg-1 to Seg-10)with lengths of 3950 bp,2904 bp,2769 bp,1978 bp,1772 bp,1638 bp,1165 bp,1142 bp,1103 bp,and 832 bp respectively,were obtained.Phylogenetic analysis showed that DH10M1019 is a TIBOV strain,with eight gene segments(Seg-1 to Seg-4,Seg-6,Seg-8 to Seg-10)clustering with known strains in the same major branch,while Seg-5 and Seg-7 formed distinct branches,independent of known reference strains,suggesting that DH10M1019 may represent a new serotype of TIBOV.Conclusions Through complete genome sequence analysis,it was confirmed that DHL10M117 and DH10M1019 are AKV and TIBOV,which provided a scientific basis for the epidemiological characterisation,pathogenicity analysis and investigation of the two viruses.

关 键 词：阿卡斑病毒 西藏环状病毒 宏基因组 序列分析 

分 类 号：S85[农业科学—兽医学]