改良支架对胚胎解冻后复苏效果及临床妊娠情况的影响  

Effect of improved scaffolds on the recovery of thawed embryos and clinical pregnancy

作　　者：谢丽松 周椿昊 岳林林 XIE Lisong;ZHOU Chunhao;YUE Linlin(Department of Reproduction,Ganzhou Women and Children's Health Care Hospital,Jiangxi Province,Ganzhou 341000,China)

机构地区：[1]江西省赣州市妇幼保健院生殖科,江西赣州341000

出　　处：《中国当代医药》2024年第35期79-82,87,共5页China Modern Medicine

基　　金：江西省卫生健康委科技计划项目(202212352)。

摘　　要：目的探讨改良支架对胚胎解冻后复苏效果及临床妊娠情况的影响。方法选取2019年4月至2023年3月赣州市妇幼保健院收治的80例行冻融胚胎移植的患者作为研究对象,均行玻璃化冷冻。按照随机数字表法分为对照组和观察组,每组40例,分别采用传统型支架(铝支架加塑料套管)、创新型支架(侧边开口的塑料支架)对冷冻胚胎进行储存,解冻后进行移植。比较两组患者的胚胎复苏效果、临床妊娠情况、液氮罐存储空间利用率和胚胎拿取时间,同时对获得临床妊娠的产妇随访至妊娠结束,对两组产妇妊娠结局及新生儿出生结局进行统计分析。结果两组患者胚胎复苏率、优质胚胎率比较,差异无统计学意义(P>0.05)。两组患者胚胎着床率、胚胎种植率、临床妊娠率、多胎率及自然流产率比较,差异无统计学意义(P>0.05)。观察组、对照组分别可盛放150、40支载杆,观察组液氮罐存储空间利用情况优于对照组。观察组胚胎拿取时间为(6.90±1.50)min,短于对照组的(11.50±2.03)min,差异有统计学意义(P<0.05)。两组产妇剖宫产率、胎膜早破率、羊水异常率、早产率、产后出血率及新生儿低出生体重率、出生缺陷率、1 min Apgar评分、5 min Apgar评分比较,差异无统计学意义(P>0.05)。结论创新型支架、传统型支架对胚胎复苏质量、临床妊娠情况、产妇妊娠结局及新生儿出生结局的影响差别不大,但创新型支架液氮罐存储空间利用情况好,胚胎定位准确,便于查找和提取,可明显缩短胚胎拿取时间,减少医疗差错,是一种值得临床推广的胚胎冷冻储存方法。Objective To investigate the effect of improved scaffolds on the recovery of thawed embryos and clinical pregnancy.Methods A total of 80 patients who underwent thawed embryos transfer from April 2019 to March 2023 in Ganzhou Women and Children's Health Care Hospital were selected as the research objects,all of whom underwent vitrification freezing.According to the random number table method,they were divided into control group and observation group,with 40 cases in each group.The frozen embryos were stored with traditional scaffolds(aluminum scaffolds plus plastic sleeves)and innovative scaffolds(plastic scaffolds with side openings),respectively,and then transplanted after thawing.The effects of embryo recovery,clinical pregnancy status,utilization rate of liquid nitrogen tank storage space and embryo retrieval time of the two groups were compared.At the same time,the pregnant women who obtained clinical pregnancy were followed up until the end of pregnancy,and the pregnancy outcome and newborn birth outcome of the two groups were statistically analyzed.Results There were no significant differences in the rates of embryo recovery and high-quality embryo between the two groups(P>0.05).There were no significant differences in the rates of embryo implantation,embryo implantation,clinical pregnancy,multiple birth and spontaneous abortion between two groups(P>0.05).Observation group and control group can hold 150 and 40 load rods,respectively.The storage space utilization of liquid nitrogen tank in observation group was better than that in control group.The embryo retrieval time in the observation group was(6.90±1.50)min,which was shorter than(11.50±2.03)min in the control group,and the difference was statistically significant(P<0.05).There were no significant differences in cesarean section rate,premature rupture rate,abnormal amniotic fluid rate,premature delivery rate,postpartum hemorrhage rate,low birth weight rate,birth defect rate,1 min Apgar score and 5 min Apgar score between the two groups(P>0.05).Conclusi

关 键 词：胚胎冷冻 胚胎移植 玻璃化冷冻 胚胎复苏 妊娠结局 

分 类 号：R714.8[医药卫生—妇产科学]