玉米皮可溶性膳食纤维对洛哌丁胺诱导小鼠便秘的作用及机制  

Effect and Mechanism of Soluble Dietary Fiber from Corn Bran on Loperamide-induced Constipation in Mice

作　　者：曾祥瑞 姜彩霞 刘晓兰 郑喜群 魏旭瑶 ZENG Xiangrui;JIANG Caixia;LIU Xiaolan;ZHENG Xiqun;WEI Xuyao(College of Food Science,Heilongjiang Bayi Agricultural University,Daqing 163319,China;National Coarse Cereals Engineering Center,Heilongjiang Bayi Agricultural University,Daqing 163319,China;Engineering Research Center of Processing and Utilization of Grain By-products and Utilization of Ministry of Education,Daqing 163319,China;Heilongjiang Key Laboratory of Corn Deep Processing Theory and Technology,College of Food and Biological Engineering,Qiqihar University,Qiqihar 161006,China;Heilongjiang Provincial Key Laboratory of Corn Processing,Qiqihar 161006,China)

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]黑龙江八一农垦大学国家杂粮工程技术中心,黑龙江大庆163319 [3]粮食副产物加工与利用教育部工程研究中心,黑龙江大庆163319 [4]齐齐哈尔大学食品与生物工程学院黑龙江省玉米深加工理论与技术重点实验室,黑龙江齐齐哈尔161006 [5]黑龙江省玉米深加工理论与技术重点实验室,黑龙江齐齐哈尔161006

出　　处：《食品工业科技》2025年第4期374-384,共11页Science and Technology of Food Industry

基　　金：国家十四五重点研发计划子课题(2021YFD2100904);黑龙江省百千万工程项目子课题(2021ZX12B09-4)。

摘　　要：本文主要研究玉米皮可溶性膳食纤维(corn bran soluble dietary fiber,CSDF)对洛哌丁胺诱导的小鼠便秘及小鼠肠道菌群变化的影响。60只BALB/c雄性小鼠随机分为正常组,模型组,CSDF低、中、高剂量组(3、6、12 g·kg^(-1)·d)和联合组(CSDF:副干酪乳杆菌=30:1(v:v)),每组10只。CSDF持续干预14 d,干预结束后以洛哌丁胺悬浊液(10 mg·kg^(-1))诱导小鼠便秘,持续7 d。排便试验结果表明:与模型组相比,CSDF干预能显著缩短便秘小鼠首粒黑便时间,6 h排便量和粪便含水量显著提高(P<0.05)。小肠推进率和结肠病理试验表明,与模型组相比,联合组小肠推进率提升了110.58%,结肠隐窝深度提升了43.67%。与模型组相比,CSDF摄入后小鼠胃肠道相关神经递质(胃动素、5-羟色胺等)水平升高,提示CSDF可改善便秘性肠道神经系统紊乱。此外,补充CSDF后,有益肠道细菌(双歧杆菌、乳杆菌和毛螺菌等)丰度上调,短链脂肪酸(short chain fatty acids,SCFAs)含量升高,L-CSDF组中典型的SCFAs(乙酸、丙酸和丁酸)分别达到了模型组的1.89、1.60和4.35倍。综上,CSDF可通过调节神经递质水平和重塑肠道菌群及代谢物水平来缓解便秘,且联合益生菌后CSDF可更有效的缓解便秘引发的结肠炎症。This study was performed to elucidate effects of corn bran soluble dietary fibers(CSDF)on loperamide-induced constipation and changes in intestinal flora in mice.Sixty male BALB/c mice were randomly divided into a normal group,a model group,CSDF low,medium,and high dose groups(3,6,and 12 g·kg^(-1)·d),and a combination group(CSDF:Lactobacillus paracasei=30:1(v:v)),with 10 mice in each group.CSDF was administered for 14 d.At the end of the intervention,constipation was induced with single dose administration of a loperamide suspension(10 mg·kg^(-1)),and mice were monitored for 7 d.Compared with the model group,CSDF intervention significantly shortened the time to the first black stool in constipated mice.The volume of defecation spanning 6 h and the water content of feces were also significantly increased(P<0.05).Small bowel propulsion rate and colon pathology experiments showed a 110.58%increase in small bowel propulsion rate,and a 43.67%increase in colonic crypt depth in the combined group compared with that of the model group.Compared to that of the model group,the levels of gastrointestinal tract-related neurotransmitters(motilin,5-hydroxytryptamine,etc.)were elevated in mice that received CSDF,suggesting that CSDF could alleviate constipation in enteric nervous system disorders.CSDF supplementation also increased the abundance of beneficial gut bacteria(Bifidobacterium,Lactobacillus,and Lachnospiraceae)and elevated levels of short chain fatty acids(SCFAs),with typical SCFAs(acetic,propionic,and butyric acids)reaching 1.89-,1.60-,and 4.35-fold higher levels,respectively,in the L-CSDF group than that in the model group.In conclusion,CSDF can alleviate constipation by regulating neurotransmitter levels and remodeling intestinal flora and metabolite levels,and its efficacy in alleviating constipation-induced colonic inflammation can be enhanced by administration in combination with probiotics.

关 键 词：玉米皮可溶性膳食纤维 副干酪乳杆菌 缓解便秘 肠道菌群 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]