基于SIRT1/PGC-1α信号通路的同仁牛黄清心丸治疗后循环缺血性眩晕大鼠作用机制探讨  

Mechanism of Tongren Niuhuang Qingxin Pills in the Treatment of Posterior Circulation Ischemic Vertigo in Rats Based on SIRT1/PGC-1αSignaling Pathway

作　　者：陈霞[1] 刘丹[1] 张思玉[1] 朱晓光[1] 李晋生[1] CHEN Xia;LIU Dan;ZHANG Siyu;ZHU Xiaoguang;LI Jinsheng(Science Research Institute of Beijing Tong Ren Tang Co.,Ltd.,Beijing 100079,China)

机构地区：[1]北京同仁堂股份有限公司科学研究所,北京100079

出　　处：《中国现代中药》2025年第1期98-104,共7页Modern Chinese Medicine

基　　金：北京市中医药科技发展资金项目(BJZYYB-2023-51)。

摘　　要：目的:探讨同仁牛黄清心丸对后循环缺血性眩晕大鼠的保护作用及机制。方法:采用手术结扎右侧颈总动脉和锁骨下动脉致大鼠右侧半脑不完全脑缺血制备后循环缺血性眩晕大鼠模型。将大鼠分为假手术组,模型组,地芬尼多组,金纳多组,同仁牛黄清心丸高(2.4 g·kg^(-1))、中(1.2 g·kg^(-1))、低(0.6 g·kg^(-1))剂量组,观察同仁牛黄清心丸对旋转刺激后循环缺血性眩晕大鼠跳台逃避潜伏期和前庭神经核血流量的影响;取大鼠右侧脑组织并测定超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)活性和丙二醛(MDA)、乳酸(Lac)含量;采用蛋白质免疫印迹(Western blot)法测定脑组织中沉默调节蛋白1(SIRT1)和增殖物激活受体γ辅激活因子-1α(PGC-1α)的蛋白质表达水平。结果:与假手术组相比,模型组大鼠跳台逃避潜伏期延长,脑前庭神经核血流量降低,脑组织中LDH活性和MDA、Lac含量提高,脑组织中SOD活性和SIRT1、PGC-1α表达水平降低。与模型组相比,同仁牛黄清心丸显著缩短大鼠跳台逃避潜伏期,增加脑前庭神经核血流量,降低大鼠脑组织中LDH活性和Lac、MDA含量,升高大鼠脑组织中SOD活性和SIRT1、PGC-1α表达水平。结论:同仁牛黄清心丸能够增加脑缺血后前庭神经核血流量,减轻模型大鼠眩晕症状,通过调控SIRT1/PGC-1α信号通路改善脑组织能量代谢,提高抗氧化能力,发挥神经保护作用。Objective:To explore the protective mechanism of Tongren Niuhuang Qingxin Pills against brain injury in posterior circulation ischemic vertigo rats.Methods:The rat model of posterior circulation ischemic vertigo was established by surgical ligation of the right common carotid artery and the subclavian artery,resulting in incomplete cerebral ischemia in the right hemisphere.The rats were randomly divided into sham operation group,model group,difenidol group,ginaton group,and Tongren Niuhuang Qingxin Pills groups at doses of 2.4,1.2,and 0.6 g·kg^(-1).The effect of Tongren Niuhuang Qingxin Pills on the latency of platform jumping and vestibular nucleus blood flow in rats with circulatory ischemic vertigo after rotational stimulation was observed.The activities of superoxide dismutase(SOD)and lactic dehydrogenase(LDH),and the content of malondialdehyde(MDA)and lactic acid(Lac)in the right brain tissue of rats were determined.The expression levels of silencing regulatory protein 1(SIRT1)and proliferator-activated receptorγcoactivator-1α(PGC-1α)in the brain tissue were determined by Western blot.Results:Compared with the sham operation group,the model group had a longer latency for jumping off the platform,lower blood flow to the vestibular nucleus,increased MDA,LDH,and Lac levels in the brain tissue,and lower SOD activity,SIRT1,and PGC-1αexpression levels in the brain tissue.Compared with the model group,Tongren Niuhuang Qingxin Pills significantly shortened the latency period for rats to jump off the platform,increased the blood flow of the vestibular nucleus,decreased LDH activity and Lac and MDA levels in rat brain tissue,increased SOD activity in rat brain tissue,and upregulated SIRT1 and PGC-1αexpression levels in rat brain tissue.Conclusion:Tongren Niuhuang Qingxin Pills can increase the blood flow of the vestibular nucleus after cerebral ischemia and alleviate vertigo symptoms in model rats,and improve brain energy metabolism and antioxidant capacity by regulating the SIRT1/PGC-1αsignaling pathway,thereb

关 键 词：同仁牛黄清心丸 后循环缺血性眩晕 沉默调节蛋白1 增殖物激活受体γ辅激活因子-1α 

分 类 号：R285.5[医药卫生—中药学]