大豆-根瘤菌共生结瘤能力鉴定培养条件的筛选  

Screening of Culture Conditions for the Identification of Nodulation Ability of Soybean-rhizobium Symbiosis

作　　者：赵圆 刘智 杨庆 王宇 王艳丽 闫龙[2] 张锴 史晓蕾[2] 刘晓燕 ZHAO Yuan;LIU Zhi;YANG Qing;WANG Yu;WANG Yanli;YAN Long;ZHANG Kai;SHI Xiaolei;LIU Xiaoyan(College of Agronomy and Biotechnology,Hebei Normal University of Science&Technology,Qinhuangdao 066004,China;Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050035,China;Hebei Seed Station,Shijiazhuang 050031,China)

机构地区：[1]河北科技师范学院农学与生物科技学院,河北秦皇岛066004 [2]河北省农林科学院粮油作物研究所,河北石家庄050035 [3]河北省种子总站,河北石家庄050031

出　　处：《华北农学报》2024年第S1期125-134,共10页Acta Agriculturae Boreali-Sinica

基　　金：河北省大豆产业技术体系(HBCT2024060204);河北省科技厅现代种业科技创新专项大豆现代种业科技创新团队(21326313D);国家大豆产业技术体系(CARS-04-PS06);河北省农林科学院科技创新专项课题资助(2022KJCXZX-LYS-7);坝上大豆种质资源引进筛选和新品系培育(2023KJCXZX-LYS-23)。

摘　　要：为探究不同环境对大豆-根瘤菌共生结瘤能力的影响,筛选适宜大豆-根瘤菌共生结瘤的培养条件,设置4种环境因素共10种处理(培养温度:22,26,30℃;培养基质:蛭石,蛭石营养土;接种时期:0 d接种,10 d接种;摇菌终浓度:OD_(600)=0.5,OD_(600)=0.9,OD_(600)=1.3),组合出36种培养条件。测定36种培养条件下大豆-根瘤菌的5个结瘤相关表型,即单株根瘤数、单株根瘤干质量、根瘤大小、叶片生长10,24 d的SPAD值,运用主成分分析、模糊数学隶属函数法和聚类分析法对各条件下大豆-根瘤菌共生结瘤能力进行综合评价和分类,建立大豆-根瘤菌结瘤能力综合评价体系,并根据综合评价D值筛选最优培养条件。结果显示:培养温度是影响大豆-根瘤菌共生结瘤的主要环境因素,不同培养条件下的单株根瘤数、单株根瘤干质量、根瘤大小、叶片生长10,24 d的SPAD值的最大值对应的培养温度分别为30,26,26,30,26℃。在本研究设置的培养条件下,大豆-根瘤菌共生结瘤体系最优培养温度为26℃;通过基于综合评价D值的多元线性回归分析和灰色关联度分析得知,单株根瘤干质量和根瘤大小与大豆-根瘤菌共生结瘤能力的相关性较强,可作为结瘤能力鉴定的重要参考指标。该研究结果建立了一个大豆-根瘤菌共生结瘤能力的综合评价体系,为选择鉴定结瘤能力的表型和培养条件提供了理论依据。In order to investigate the impact of different environments on the symbiotic nodulation ability of soybean-rhizobia and to screen suitable culture conditions for symbiotic nodulation,this study established 10 treatments based on 4 environmental factors(culture temperatures:22,26,30℃;culture substrates:vermiculite,vermiculiteand nutrient soil;inoculation periods:0 day post-inoculation,inoculation at 10 days;final bacterial concentration:OD_(600)=0.5,OD_(600)=0.9,OD_(600)=1.3),resulting in 36 different culture conditions.Five nodulation-related phenotypes of soybean-rhizobia were measured under these conditions,including the number of nodules per plant,nodule dry weight per plant,nodule size,SPAD of leaf growth at 10 and 24 days post-inoculation(DPI).Principal component analysis,fuzzy mathematics membership function method,and cluster analysis were employed to comprehensively evaluate and classify the symbiotic nodulation ability of soybean-rhizobia under different conditions.A comprehensive evaluation system for the nodulation ability of soybean-rhizobia was established,and the optimal culture conditions were selected based on the comprehensive evaluation index D value.The results showed that culture temperature was the primary environmental factor influencing the symbiotic nodulation of soybean-rhizobia.The maximum values of the number of nodules per plant,nodule dry weight per plant,nodule size,SPAD of leaf growth at 10 and 24 days post-inocubion corresponded to culture temperatures of 30,26,26,30 and 26℃,respectively,under,different culture conditions.Under the culture conditions set,the optimal culture temperature for the symbiotic nodulation of soybean-rhizobia was 26℃.Multivariate linear regression analysis and grey relational analysis based on the comprehensive evaluation index D value indicated that nodule dry weight and nodule size had a strong correlation with the symbiotic nodulation ability of soybean-rhizobia,serving as important reference indicators for nodulation ability identification.The re

关 键 词：大豆 根瘤菌 共生培养 结瘤能力 综合评价 

分 类 号：S565.03[农业科学—作物学]