葡萄糖转运蛋白3在噪声诱导听觉损害中的作用研究  

The Role of Glucose Transporter 3 in Noise-induced Hearing Loss

作　　者：李重慧 郭瑞 龚树生[1,2] 柳柯[1,2] LI Chonghui;GUO Rui;GONG Shusheng;LIU Ke(Department of Otolaryngology Head and Neck Surgery,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院耳鼻咽喉头颈外科,北京100050 [2]首都医科大学耳聋疾病临床诊疗与研究中心

出　　处：《中华耳科学杂志》2025年第1期95-100,共6页Chinese Journal of Otology

基　　金：国家自然科学基金项目(82371151,82071037,82301298);北京市科委-教委联合基金项目(KZ20231002542);首都医学科学创新中心科研培育项目资助(CX24PY14)。

摘　　要：目的探索葡萄糖转运蛋白3(glucose transporter 3,Glut3)在噪声暴露后不同时间点表达量变化。方法选取C57BL/6J小鼠,随机分为对照组10只和噪声暴露(noise exposure,NE)组18只。对照组小鼠采用Western blot检测、耳蜗中轴冷冻切片、耳蜗基底膜铺片及免疫荧光染色来明确耳蜗中Glut3表达以及定位。噪声暴露组小鼠给予白噪声115 dB SPL暴露4 h,构建永久性阈值偏移模型(permanent threshold shift,PTS)小鼠。对该组其中12只小鼠进行噪声暴露后即刻、1 h和24 h取材,每组4只,进行耳蜗基底膜铺片及免疫荧光染色观察Glut3表达量是否发生变化。对该组其余6只小鼠在噪声暴露后第7、14、21和28 d进行听性脑干反应(auditory brainstem response,ABR)检测,来确保造模成功。结果Glut3在耳蜗组织中广泛表达。与对照组相比,噪声后即刻取材组Glut3表达量明显升高,具有统计学意义(P<0.05),而噪声后1 h组和24 h组Glut3荧光表达量无明显变化(P>0.05)。结论Glut3在噪声后即刻表达明显升高,对噪声发挥快速应答和能量补充作用。Objective To report changes of glucose transporter 3(Glut3)at different time points after noise exposure.Methods C57BL/6J mice were selected and randomly divided into control group(10 mice)and noise exposure(NE)group(18 mice).In the control group,expression and localization of Glut3 in the cochlea were determined by Western blot,cochlea frozen sections,basilar membrane preparation and immunofluorescence staining.Mice in the NE group were subjected to white noise at 115 dB SPL for 4 h to induce permanent threshold shift(PTS),and sampled immediately,at 1 h or 24 h after noise(n=4 in each group).Cochlear basilar membrane sections with immunofluorescence staining were used to detect changes in Glut3 expression.The remaining 6 mice in this group were subjected to auditory brainstem response(ABR)tests at 7,14,21 and 28 d after noise modeling to verify successful modeling.Results Glut3 was widely expressed in cochlear tissues.Compared with the control group,the level of Glut3 expression was higher immediately after noise exposure.Conclusion Glut3 expression is significantly elevated immediately after noise exposure,likely playing a role of rapid response to noise and enhanced energy supply.

关 键 词：葡萄糖转运蛋白Glut3 噪声性耳聋 听觉损害 耳蜗 

分 类 号：R76[医药卫生—耳鼻咽喉科]