海洋本草软珊瑚共附生真菌Aspergillus terreus EGF7-0-1中γ-芳环丁烯内酯类化合物研究(Ⅰ)  

γ-Aromatic butenolides lignans from soft coral-associated symbiotic and epiphytic fungi Aspergillus terreus EGF7-0-1(I)

作　　者：何悦铭 赵立凝 陈欣琪 何嘉鸿 樊浩 陈乐怡 张翠仙[1] 何细新[1] HE Yueming;ZHAO Lining;CHEN Xinqi;HE Jiahong;FAN Hao;CHEN Leyi;ZHANG Cuixain;HE Xixin(School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Library,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学图书馆,广东广州510006

出　　处：《热带海洋学报》2025年第1期146-153,共8页Journal of Tropical Oceanography

基　　金：国家自然科学基金项目(82273845);广东省自然资源厅专项(GDNRC[2023]37、GDNRC[2020]039、GDNRC[2021]048);广东省大学生创新创业项目(202310572224、202310572291)。

摘　　要：为了从海洋微生物中寻找结构新颖的次生代谢产物,本文采用大米培养基对海洋本草软珊瑚共附生真菌Aspergillus terreus EGF7-0-1进行规模发酵,运用薄层色谱、柱层析和高效液相色谱等多种色谱技术对发酵产物进行分离纯化,通过核磁共振波谱、高分辨质谱和旋光光谱等多种光谱学方法,并结合文献数据比对确定了8个γ-芳环丁烯内酯类化合物:butyrolactonesⅠ—Ⅴ(1—5)、aspernolide E(6)、asperteretal D(7)和terrusnolide A(8)。首次对化合物1—8进行体外抑制酪氨酰-DNA磷酸二酯酶I(tyrosyl-DNA phosphodiesterase 1,TDP1)活性测试,均无抑制作用。本研究为TDP1抑制剂的发现提供借鉴。In order to discover the novel and bioactive secondary metabolites,the soft coral-associated symbiotic and epiphytic fungi Aspergillus terreus EGF7-0-1 were fermented in rice media.The secondary metabolites were isolated using various chromatographic technologies,yielding eightγ-aromatic butenolide lignans.Based on nuclear magnetic resonance(NMR),high resolution electrospray ionization mass spectroscopy(HR-ESI-MS)and comparison with the literature,their structures were determined to be butyrolactones I-V(1-5),aspernolide E(6),asperteretal D(7)and terrusnolide A(8).In this study,the inhibitory activity of these compounds against TDP1(Tyrosyl-DNA phosphodiesterase 1)in vitro was studied for the first time,but it was unfortunate that compounds 1-8 did not show any inhibitory abilities against TDP1.This study provides theoretical data for the discovery of TDP1 inhibitors.

关 键 词：海洋本草软珊瑚 Aspergillus terreus γ-芳环丁烯内酯类 结构鉴定 TDP1抑制剂 

分 类 号：O629[理学—有机化学]