基于转录组和基因组数据筛选剑白香猪两头乌毛色的候选基因  

Screening of Candidate Genes for Two-end Black Coat Color in Jianhe White Xiang Pigs Based on Transcriptome and Genome Data

作　　者：胡紫平 苏艳芳 宗文成 牛乃琪 张龙超 王源 HU Ziping;SU Yanfang;ZONG Wencheng;NIU Naiqi;ZHNAG Longchao;WANG Yuan(College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China;Institute of Animal Science of CAAS,Beijing 100193,China)

机构地区：[1]青岛农业大学动物科技学院,青岛266109 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国畜牧兽医》2025年第1期13-24,共12页China Animal Husbandry & Veterinary Medicine

基　　金：国家重点研发计划(2021YFD1200303);国家生猪产业技术体系(CARS-35);中国农业科学院科技创新工程(ASTIP-IAS02)。

摘　　要：[目的]剑白香猪的毛色表型多为两头乌,但影响其毛色表型的候选基因鲜见报道。试验旨在筛选影响剑白香猪两头乌毛色的候选基因。[方法]基于剑白香猪黑、白皮肤组织的转录组数据进行差异表达基因筛选,同时结合138头剑白香猪与130头北京黑猪50K芯片数据(Porcine SNP50 BeadChip)进行选择信号分析,通过GO功能和KEGG通路分析筛选影响剑白香猪毛色的候选基因。[结果]在转录组分析中,554个基因在黑、白毛色皮肤组织中差异表达,其中295个上调,259个下调。共筛选到6个GO条目和2条KEGG通路,获得TYR、TYRP1、DCT、PLCB4等12个与毛色形成相关的候选基因。基于基因组数据进行群体间遗传分化指数(Fst)分析共筛选到3个GO条目、4条KEGG通路中PLCB4、FZD8、WNT2等32个候选基因可能参与剑白香猪毛色形成过程。[结论]综合选择信号和转录组差异分析,两者共同筛选到的基因为PLCB4,可将其作为影响剑白香猪两头乌毛色表型的重要候选基因,为今后剑白香猪毛色形成相关分子调控机制研究提供参考。[Objective]The coat color phenotype of Jianhe White Xiang pigs is mostly two-end black,but the candidate genes affecting its coat color phenotype have not been reported.This study was aimed to screen the candidate genes affecting the two-end black coat color of Jianhe White Xiang pigs.[Method]Based on the transcriptome data of black and white skin tissues of Jianhe White Xiang pigs,differentially expressed gene screening was performed,and at the same time,the selective signals were analyzed using the data from 138 Jianhe White Xiang pigs and 50K chip of 130 Beijing Black pigs(Porcine SNP50 BeadChip),and the candidate genes affecting coat color were screened by GO function and KEGG pathway analysis.[Result]The results showed that 554 genes were differentially expressed in black and white coat color skin tissues in the transcriptome analysis,of which 295 were up-regulated and 259 were down-regulated.A total of 6 GO terms and 2 KEGG pathways,and a total of 12 candidate genes,such as TYR,TYRP 1,DCT and PLCB 4,related to the formation of hair color were screened.Based on the genome data,F st analysis was performed,and a total of 3 GO terms and 4 KEGG pathways were screened,32 other candidate genes,such as PLCB 4,FZD 8 and WNT 2,might be involved in the process of hair color formation in Jianhe White Xiang pigs.[Conclusion]Through comprehensive selection signal and transcriptome differential analysis,PLCB 4 gene was identified as an important candidate gene that affected the phenotype of two-end black coat color in Jianhe White Xiang pigs.The results provided a reference for future molecular regulatory mechanisms related to coat color formation in Jianhe White Xiang pigs.

关 键 词：剑白香猪 转录组 基因组 毛色 候选基因 

分 类 号：S813.3[农业科学—畜牧学]