高精料饲粮中添加辣蓼对绵羊瘤胃发酵参数和瘤胃菌群的影响  

Effects of Adding Polygonum hydropiper L.to High-concentrate Diet on Fermentation Parameters and Flora of Rumen in Sheep

作　　者：于天力 何振莲 韩怡静 任靖宇 夏呈强 裴彩霞[1] YU Tianli;HE Zhenlian;HAN Yijing;REN Jingyu;XIA Chengqiang;PEI Caixia(College of Animal Sciences,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学动物科学学院,太谷030801

出　　处：《中国畜牧兽医》2025年第1期205-214,共10页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然基金项目(31972590);山西省自然科学研究面上项目(202303021221096)。

摘　　要：[目的]试验旨在研究高精料饲粮中添加辣蓼对绵羊瘤胃发酵参数、菌群数量、乳酸代谢及酶活性的影响。[方法]选取40只体重(23.26±0.33)kg的3月龄杜寒杂交公羔,随机分为4组,每组10只。基础饲粮精粗比为75∶25,辣蓼添加量分别为0(对照组)、5(L1组)、10(L2组)和15 g/d(L3组)。预饲期15 d,正式期60 d,共计75 d。正式期结束当天晨饲3 h后,对所有试验羊进行瘤胃液采集,用于后续瘤胃发酵参数、瘤胃微生物酶活、瘤胃乳酸代谢和瘤胃菌群的测定。[结果](1)L3组绵羊瘤胃液中总挥发性脂肪酸(TVFA)含量显著高于对照组和L1组(P<0.05),丙酸含量显著高于其他3组(P<0.05),乙丙比显著低于对照组,氨态氮(NH_(3)-N)含量显著低于L1组和对照组(P<0.05),微生物蛋白(MCP)含量显著高于L1组和对照组(P<0.05);L2组绵羊瘤胃液中丙酸和MCP含量显著高于对照组(P<0.05),乙丙比和NH_(3)-N含量显著低于对照组(P<0.05);L1组绵羊瘤胃液中NH_(3)-N含量显著低于对照组(P<0.05)。(2)L2和L3组绵羊瘤胃液中纤维二糖酶活性显著高于对照组(P<0.05),蛋白酶活性显著低于对照组(P<0.05);L3组绵羊瘤胃液中蛋白酶活性还显著低于L1组(P<0.05),α-淀粉酶活性显著高于L1组和对照组(P<0.05)。(3)L3组绵羊瘤胃液中乳酸含量显著低于对照组和L1组(P<0.05),乳酸脱氢酶活性显著低于其他3组(P<0.05);3个试验组绵羊瘤胃液中丙酮酸含量和丙酮酸激酶活性均显著高于对照组,且L3组显著高于L2和L1两组(P<0.05)。(4)L3组绵羊瘤胃液中总产甲烷菌数量显著低于对照组(P<0.05),总原虫和栖瘤胃普雷沃氏菌数量显著低于L1组和对照组(P<0.05),白色瘤胃球菌数量显著高于对照组(P<0.05),嗜淀粉瘤胃杆菌数量显著高于L1组和对照组(P<0.05);L2组绵羊瘤胃液中总原虫和栖瘤胃普雷沃氏菌数量均显著低于对照组(P<0.05),嗜淀粉瘤胃杆菌数量显著高于对照组(P<0.05)。[结论]高精料饲粮中添�[Objective]The experiment aimed to investigate the effects of adding Polygonum hydropiper L.to high-concentrate diet on rumen fermentation parameters,microbial populations,lactic acid metabolism,and enzyme activity in sheep.[Method]Forty 3-month-old Duhan hybrid male sheeps with an average weight of(23.26±0.33)kg were selected and randomly divided into four groups with 10 sheeps per group.Lambs were fed individually with a crude-to-processed diet ratio of 75∶25.The levels of Polygonum hydropiper L.supplementation were 0(control),5(L1),10(L2),and 15 g/d(L3).After a 15-day adaptation period,a 60-day experimental period was conducted,lasting 75 days.Rumen fluid samples were collected from all lambs 3 hours after morning feeding at the end of the experimental period for subsequent analysis of rumen fermentation parameters,microbial enzyme activities,lactic acid metabolism,and microbial populations.[Result]①The total volatile fatty acid(TVFA)content in rumen fluid of sheep in group L3 was significantly higher than that in control group and L1 group(P<0.05),the propionic acid content was significantly higher than that in the other three groups(P<0.05),the ratio of ethylene to propylene was significantly lower than that in control group,and the ammonia nitrogen(NH_(3)-N)content was significantly lower than that in L1 group and control group(P<0.05).The content of microbial protein(MCP)was significantly higher than that in L1 group and control group(P<0.05).The contents of propionic acid and MCP in rumen fluid of sheep in L2 group were significantly higher than those in control group(P<0.05),the ratio of ethylene to propylene and the content of NH_(3)-N in L2 group were significantly lower than those in control group(P<0.05),the content of NH_(3)-N in rumen fluid of sheep in L1 group was significantly lower than that in control group(P<0.05).②The activity of cellobiase in rumen fluid of sheep in L2 and L3 groups was significantly higher than that in control group(P<0.05),and the activity of protease was significan

关 键 词：高精料饲粮 辣蓼 绵羊 瘤胃发酵 乳酸 

分 类 号：S826.92[农业科学—畜牧学]