细菌培养液中卡那霉素的酶联免疫试剂盒检测法  

Enzyme-Linked Immunosorbent Assay Kit for Detection of Kanamycin in Bacterial Culture Broth

作　　者：邓兴朝 曾祥富 林金昌 冯滨 金洪伟 DENG Xingchao;ZENG Xiangfu;LIN Jinchang;FENG Bin;JIN Hongwei(Shenzhen Reagent Technology Co.,Ltd.,Shenzhen 518133,China;Shenzhen Guangming District Center for Disease Control and Prevention,Shenzhen 518106,China)

机构地区：[1]深圳容金科技有限公司,广东深圳518133 [2]深圳市光明区疾病预防控制中心,广东深圳518106

出　　处：《化学与生物工程》2025年第2期66-68,共3页Chemistry & Bioengineering

基　　金：深圳市科创委基础研究专项(自然科学基金)面上项目(JCYJ20210324140406018,JCYJ20230807152459009);深圳市光明区软科学研究项目(2021R01146)。

摘　　要：采用自制酶联免疫试剂盒检测细菌培养液中的卡那霉素含量。方法学验证结果表明,酶联免疫试剂盒能够准确检测细菌培养液中的卡那霉素含量,标准品浓度在0.0~16.2μg·L^(-1)范围内与吸光度线性关系良好,线性系数为0.9959,加标回收率为97.0%~102.8%,批内变异系数为3.66%,批间变异系数为5.05%,满足酶联免疫试剂盒要求。We used a self-made enzyme-linked immunosorbent assay kit to detect the content of Kanamycin in bacterial culture broth.The methodological validation results show that the enzyme-linked immunosorbent assay kit can accurately detect the content of Kanamycin in bacterial culture broth.The standard concentration has a good linear relationship with absorbance within the range of 0.0-16.2μg·L^(-1),with a linear coefficient of 0.9959,a recovery of 97.0%-102.8%,an intra-batch variation coefficient of 3.66%,and an inter-batch variation coefficient of 5.05%,which meet the requirements of the enzyme-linked immunosorbent assay kit.

关 键 词：细菌培养液 卡那霉素 酶联免疫 定量检测 

分 类 号：O65[理学—分析化学] Q81[理学—化学]