基于基因表达综合数据库和药物表达谱模式探究高胆固醇血症的潜在中药  

Exploration of the potential Chinese medicine for hypercholesterolemia based on gene expression omnibus database and drug expression pattern

作　　者：刘思莹 徐丹[2] 张玲[3] 倪雪妍 仲丽丽[4] LIU Siying;XU Dan;ZHANG Ling;NI Xueyan;ZHONG Lii(College of Veterinary Medicine,Heilongjiang Agricultural Engineering Vocational College,Harbin,Heilongjiang,150070,China;Pharmacy Department,the Second Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin,Heilongjiang,150001,China;Clinical Laboratory,the First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin,Heilongjiang,150040,China;Department of Pathology,the First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin,Heilongjiang,150040,China)

机构地区：[1]黑龙江农业工程职业学院动物医学学院,黑龙江哈尔滨150070 [2]黑龙江中医药大学附属第二医院药剂科,黑龙江哈尔滨150001 [3]黑龙江中医药大学附属第一医院检验科,黑龙江哈尔滨150040 [4]黑龙江中医药大学附属第一医院病理科,黑龙江哈尔滨150040

出　　处：《甘肃中医药大学学报》2024年第6期35-45,共11页Journal of Gansu University of Chinese Medicine

基　　金：黑龙江省自然科学基金联合引导项目(LH2022H068);黑龙江中医药大学研究生创新科研项目立项基金(2022yjscx012);黑龙江农业工程职业学院人才引进科研启动项目(14)。

摘　　要：目的采用生物信息学方法探究高胆固醇血症相关差异基因和潜在的治疗中药。方法通过基因表达综合(GEO)数据库下载高胆固醇血症的基因表达谱芯片,借助R软件分析差异基因;采用Cytoscape 3.9.1软件构建差异基因相互作用可视化网络,利用DAVID数据库对关键核心基因进行基因本体(GO)富集分析和京都基因与基因组百科全书(KEGG)通路富集分析;采用关联图谱(CMap)数据库预测有效小分子化合物,通过中药系统药理学数据库与分析平台(TCMSP)反向匹配,筛选出防治高胆固醇血症的潜在中药,并进行文献考证。结果筛选出关键差异基因108个,主要参与类固醇生物合成信号通路、磷脂酰肌醇3-激酶/蛋白激酶B(PI3K-Akt)信号通路、Rap1信号通路、剪接体信号通路、吞噬体信号通路;通过CMap数据库比对分析,获得9个化合物分子,其对应候选中药20味。结论本研究筛选出了高胆固醇血症的关键致病基因和有效小分子化合物,并匹配到了对应的高频中药,可为探索中药组方配伍及中医药治疗高胆固醇血症提供新思路。Objective To explore differential genes associated with hypercholesterolemia and identify potential therapeutic Chinese medicine through bioinformatics approaches.Methods The gene expression profile of hypercholesterolemia was downloaded from the gene expression omnibus(GEO)database,and differential genes were analyzed using R software.The differential gene interaction visualization network was constructed using Cytoscape 3.9.1 software,and the key core genes were subjected to gene ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis using the DAVID database.The effective small molecule compounds were predicted using the cMap database,and the potential Chinese herbs for the treatment of hypercholesterolemia were screened by reverse matching with the TCMSP database and literature verification.Results A total of 108 key differential genes were identified,mainly involved in the steroid biosynthesis signaling pathway,PI3K-Akt signaling pathway,Rap1 signaling pathway,spliceosome signaling pathway,and phagosome signaling pathway.9 compound molecules were obtained through cMap database comparison analysis,and their corresponding candidate Chinese herbs were 20.Conclusion This study identified the key pathogenic genes and effective small molecule compounds for hypercholesterolemia and matched them with frequently used Chinese herbs,providing new insights into exploring the formula matching and treatment of hypercholesterolemia with traditional Chinese medicine.

关 键 词：高胆固醇血症 差异基因 中药 生物信息学 

分 类 号：R589.2[医药卫生—内分泌] R282.7[医药卫生—内科学]