基于ISSR标记的广西桄榔种质资源遗传多样性及亲缘关系分析  

Genetic diversity and genetic relationship analysis of Arenga pinnata germplasm resources based on ISSR markers

作　　者：杨海霞 兰秀 李恒锐 梁振华 马仙花 韦婉羚 黄珍玲 陈会鲜 YANG Haixia;LAN Xiu;LI Hengrui;LIANG Zhenhua;MA Xianhua;WEI Wanling;HUANG Zhenling;CHEN Huixian(South Asian Tropical Agricultural Science Research Institute of Guangxi,Chongzuo 532415,Guangxi,China)

机构地区：[1]广西南亚热带农业科学研究所,广西崇左532415

出　　处：《经济林研究》2024年第4期41-50,共10页Non-wood Forest Research

基　　金：广西自然科学基金项目(2020GXNSFBA297054);广西农科院稳定资助科研团队项目(桂农科2021YT158);广西农业科学院基本科研业务专项(桂农科2023YM29)。

摘　　要：【目的】为桄榔种质资源的保护和可持续开发利用提供参考。【方法】采用试剂盒方法,选择健康、新鲜、表现良好的嫩叶,提取广西18个桄榔居群的201份样品基因组DNA,通过ISSR分子标记技术,利用10条清晰明亮、多态性好的ISSR引物对桄榔样品基因组DNA进行PCR扩增。使用POPGENE32软件计算引物的多态性指标,用NTSYS软件中的UPGMA法构建居群间的聚类图,分析评价广西桄榔种质资源遗传多样性与亲缘关系。【结果】10条引物共扩增出150个位点,所有位点均具有多态性,多态性比率为100%,等位基因数量平均值为1.5522,有效等位基因数量平均值为1.2786,Shannon’s遗传多样性信息指数平均值为0.2578,Nei’s基因多样性指数平均值为0.1681。弄岗居群具有最高的遗传多样性(Nei’s基因多样性指数为0.2144),南宁市人民公园居群的遗传多样性最低(Nei’s基因多样性指数为0.1047)。201份供试样本的遗传相似性系数为0.8338~0.9832,平均值为0.9162。其中,崇左市天等县居群与南宁市人民公园居群之间遗传相似性系数最低,遗传差异性最大,亲缘关系最远;龙州县水口镇居群与龙州县武德乡居群之间遗传相似性系数最大,遗传差异性最小,亲缘关系最近。聚类分析结果显示,在遗传相似性系数为0.928时,可将18个居群分成5个类群。【结论】广西桄榔遗传多样性水平较高,ISSR分子标记在桄榔种质间有丰富的多态性,能较好地揭示桄榔居群间的亲缘关系,适用于桄榔遗传多样性研究。【Objective】In order to analysis the genetic diversity level of Guangxi Arenga pinnata,clarify the genetic relationship between the different A.pinnata,which provided a reference for the development utilization and protection of A.pinnata germplasm resources.【Method】The healthy,fresh and well-performing tender leaves were selected,and the genomic DNAs of 201 samples from 18 A.pinnata populations in Guangxi province were extracted using DNA Kit.Ten clearly amplified and highly polymorphic ISSR primers were used to PCR polymorphism amplification the genomic DNAs of A.pinnata samples by ISSR molecular marker technology,the percentage of polymorphic loci,the indexes of Nei’s genetic diversity and Shannon information were estimated by POPGENE32,a cluster analysis graph among cultivars was built using and NTSY-pc software,discuss and analysis the genetic diversity and relationship of germplasm resources.Based on the resource status of A.pinnata genetic diversity and the genetic structure of corresponding populations,conservation strategies for A.pinnata germplasm resources were discussed.【Result】Ten primers amplified a total of 150 sites,including 150 polymorphic sites,and the polymorphism site ratio was 100%,the average number of observed alleles was 1.5522,with the average number of effective alleles of 1.2786,the average value of Shannon information index(I)was 0.2578,the average Nei’s gene diversity index(He)was 0.1681.The Nonggang population had the highest genetic diversity(He=0.2144),and the People’s Park population had the lowest genetic diversity(He=0.1047).Cluster analysis by UPGMA method showed that the genetic similarity coefficient of the 201 test materials ranged from 0.8338 to 0.9832,with an average of 0.9162,among that,Tiandeng county Chongzuo and the People’s Park populations had the smallest genetic similarity coefficient,the genetic difference was the greatest and the kinship was the distantest;The coefficient of genetic similarity between Longzhou county Shuikou town and Wude town

关 键 词：桄榔 ISSR分子标记 遗传多样性 聚类分析 

分 类 号：S602.4[农业科学—园艺学]