植物生长调节剂对青钱柳光合积累及黄酮合成的影响  

Effect of plant growth regulators on the photosynthetic accumulation and flavonoid synthesis of Cylocarya paliurus

作　　者：赵睿智 徐道炜[1,2] 何中声[1,2] 刘金福[1,2] 郭相亿 李伟森 张朝鹏 ZHAO Ruizhi;XU Daowei;HE Zhongsheng;LIU Jinfu;GUO Xiangyi;LI Weisen;ZHANG Chaopeng(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Key Lab Fujian University Ecology&Resource Statistics,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Niumulin Nature Reserve in Yongchun County,Quanzhou 362617,Fujian,China)

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建农林大学生态与资源统计福建省高校重点实验室,福建福州350002 [3]永春县牛姆林省级自然保护区,福建泉州362617

出　　处：《经济林研究》2024年第4期99-108,共10页Non-wood Forest Research

基　　金：福建省科技计划星火项目(2023S0039)。

摘　　要：【目的】为青钱柳矮化经济林推广提供参考。【方法】以1年生青钱柳实生苗为研究对象,选用矮壮素(500、1000、1500、2000 mg/L)、三碘苯甲酸(50、100、150、200 mg/L)、芸苔素内酯(0.05、0.10、0.15、0.20 mg/L)3种生长调节剂,分别设置4种质量浓度水平,喷施植株叶面,以清水CK处理为对照,探究不同植物生长调节剂处理下青钱柳幼苗光合积累和黄酮合成相关指标的变化规律,并采用主成分分析法,筛选适合青钱柳幼苗栽培的最优植物生长调节剂种类和浓度组合。【结果】3种植物生长调节剂均能够提高青钱柳幼苗叶片的叶绿素相对含量,500 mg/L矮壮素溶液处理的效果最为显著,比CK处理提高了35.5%。但施用矮壮素会抑制青钱柳幼苗叶片的可溶性糖、可溶性淀粉和非结构性碳水化合物的合成;三碘苯甲酸溶液的处理效果则相反,叶片可溶性糖和非结构性碳水化合物含量均在50 mg/L三碘苯甲酸溶液处理下达最大,比CK处理分别提高了71.6%和29.1%;叶片可溶性淀粉含量在150 mg/L三碘苯甲酸溶液处理下达最大,比CK处理提高了21.7%。施用植物生长调节剂会影响青钱柳幼苗光合与非光合组织生物量的比值,中间浓度的生长调节剂溶液更有利于光合与非光合组织生物量比值的提高。黄酮合成方面,3种植物生长调节剂均在一定程度上提高了青钱柳黄酮合成的2种关键酶的活性,即苯丙氨酸解氨酶和查尔酮合成酶,进而显著提高了青钱柳幼苗叶片的总黄酮含量,100 mg/L三碘苯甲酸处理下叶片总黄酮含量最高,比CK处理提高了34.9%;苯丙氨酸解氨酶和查尔酮合成酶的活性分别在1500 mg/L矮壮素溶液处理和0.20 mg/L芸苔素内酯溶液处理下达到最大。【结论】矮壮素、三碘苯甲酸和芸苔素内酯对青钱柳幼苗的光合积累及黄酮合成均有一定的调控作用,且大多为促进作用,其中150 mg/L三碘苯甲酸溶液的促进效果最�【Objective】The objective of this study is to provide a reference for the promotion of dwarf economic forests in Cylocarya paliurus.【Method】The 1-year-old seedlings were selected as the research object,and three growth regulators,four mass concentration levels,which were chlormequat chloride(500,1000,1500,2000 mg/L),triiodobenzoic acid(50,100,150,200 mg/L),brassinolide(0.05,0.10,0.15,0.20 mg/L),were set respectively,the foliar fertilizer was sprayed on the plant leaves.Using clean water treatment as a control(CK),and the changes of photosynthetic accumulation and flavonoid synthesis related indexes of C.paliurus seedlings under different plant growth regulator treatments were explored.The principal component analysis was used to screen the optimal combination of species and concentration suitable for the cultivation of C.paliurus seedlings.【Result】Three plant growth regulators could increase the relative chlorophyll content of leaves of C.paliurus seedlings,and the 500 mg/L chlormequat chloride treatment had the most significant effect,which was 35.5%higher than that of CK treatment.However,the application of chlormequat chloride inhibited the synthesis of soluble sugar,soluble starch and non-structural carbohydrates in the leaves of C.paliurus seedlings,while the effect of triiodobenzoic acid treatment was the opposite,and the content of soluble sugars and non-structural carbohydrates in leaves reached the maximum in 50 mg/L triiodobenzoic acid treatment,which was 71.6%and 29.1%higher than that of CK,respectively.The soluble starch content of leaves reached the maximum in 150 mg/L triiodobenzoic acid treatment,which was 21.7%higher than that of CK treatment.The application of plant growth regulators would affect the ratio of photosynthetic to non-photosynthetic tissue biomass of C.paliurus seedlings,and the intermediate concentration of growth regulators was more conducive to the increase of the ratio of photosynthetic to non-photosynthetic tissue biomass.In terms of flavonoid synthesis,all three plan

关 键 词：青钱柳 植物生长调节剂 叶绿素含量 光合积累 黄酮 

分 类 号：S601[农业科学—园艺学]