甘肃武威地区蜱类携带潜在新病毒的宏转录组研究  

META-TRANSCRIPTOMIC DISCOVERY FOR EMERGINGTICK-BORNE VIRUSES IN WUWEI, GANSU PROVINCE

作　　者：杨佶虎 黄琳 刘顺帅 黄爱霞[3] 刘义文[3] 江佳富 YANG Ji-hu;HUANG Lin;LIU Shun-shuai;HUANG Ai-xia;LIU Yi-wen;JIANG Jia-fu(Department of Epidemiology and Biostatistics,School of Public Health,Anhui Medical University,Hefei 230032,Anhui,China;State Key Laboratory of Pathogen and Biosecurity,Academy of Military Medical Sciences,Beijing 100071,China;Wuwei Center for Disease Control and Prevention,Wuwei 733000,Gansu,China)

机构地区：[1]安徽医科大学公共卫生学院流行病与卫生统计学系,合肥230032 [2]军事科学院军事医学研究院,病原微生物生物安全全国重点实验室,北京100071 [3]甘肃省武威市疾病预防控制中心,甘肃武威733000

出　　处：《寄生虫与医学昆虫学报》2024年第4期216-223,共8页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家自然科学基金重点支持项目(U2002219)。

摘　　要：目的 监测甘肃省蜱类自然携带病毒状况,明确丝绸之路经济带蜱媒病毒病传播风险。方法 选择甘肃省黄土高原、青藏高原和蒙新高原交汇的武威地区为样点,采集游离及寄生蜱样本,经形态鉴定后利用宏转录组测序技术获得病毒组数据,进而组装病毒序列并进行注释。依据序列系统发育分析探索蜱类自然携带病毒的多样性,发现潜在新病毒。结果 共获得武威地区蜱类3属5种98只,包括森林革蜱Dermacentor silvarum、亚洲璃眼蜱Hyalomma asiaticus、草原革蜱D.nuttalli、盾糙璃眼蜱Hy.scupense和柯洛宁血蜱Haemaphysalis kolonini。从中获得丰度最高的为楚病毒科(Chuviridae)的芈病毒属Mivirus;内罗病毒科(Nairoviridae)正内罗病毒属Orthonairovirus和痘病毒科(Poxviridae)正痘病毒属Orthopoxvirus次之。组装后序列比对为博乐蜱病毒1(Bole tick virus 1)、博乐蜱病毒3(Bole tick virus 3)、昌平蜱病毒1(Changping tick virus 1)、泰顺蜱病毒(Taishun tick virus)序列。同时,发现一种新的黄病毒科(Flaviviridae)病毒成员,与最接近的未定种黄病毒相似度为96.92%,暂命名为“类博乐蜱病毒4(Bole-like tick virus 4)”,有待进一步分离和鉴定。结论 甘肃省蜱类携带多种蜱媒病毒,可能存在新病毒,应持续、有效地开展监测和预防工作。Objective The aim of the paper is to monitor the natural infection of the viruses in ticks from the Gansu Province and elicit the risk of tick-borne viral disease transmission in the Silk Road Economic Zone.Methods The Wuwei area,where the Loess Plateau,the Tibetan Plateau and the Meng-Xin Plateau converge,was selected as a surveillance site to collect off-host or parasitic tick samples and detect the potential viruses harbored in them with meta-transcriptomic sequencing.The clean reads were assembled and annotated to explore the diversity of viruses under the genome phylogenetic analyses,identifying potential novel viruses.Results A total of 98 ticks of 5 species in 3 genera were sampled,including Dermacentor silvarum,Dermacentor nuttalli,Hyalomma asiaticum,Hyalomma scupense and Haemaphysalis kolonini.Among these tick samples,Mivirus shows the highest abundance,followed by Orthopoxvirus and Orthonairovirus.The assembled viral sequences were identical to Bole tick virus 1,Bole tick virus 3,Changping tick virus 1 and Taishun tick virus.Meanwhile,a novel subspecies of Bole tick virus 4 with 96.92%identity to the closest Flaviviridae sp.was detected and provisionally named"Bole-like tick virus 4"with pending characterization by isolation or other approaches.Conclusions The occurrence of diverse tick-borne viruses and potential novel viruses in the surveillance sites indicates that surveillance and preventive measures are warranted.

关 键 词：宏转录组 蜱媒病毒 甘肃 蜱 类博乐蜱病毒 

分 类 号：R384.4[医药卫生—医学寄生虫学]