2022年“一带一路”部分口岸鼠类及其携带钩端螺旋体调查研究  

GENOTYPES OF LEPTOSPIROSIS IN RODENTS FROMVARIOUS PORTS ALONG THE BELT AND ROAD IN 2022

作　　者：郭惠琳 潘娟[2] 张晓晨 赵志亮 王董 孙志文 田洁 张晓龙 宋悦谦 GUO Hui-lin;PAN Juan;ZHANG Xiao-chen;ZHAO Zhi-liang;WANG Dong;SUN Zhi-wen;TIAN Jie;ZHANG Xiao-long;SONG Yue-qian(Science and Technology Research Center of China Customs,Beijing 100026,China;Tianjin International Travel Healthcare Center,Tianjin 300456,China;Shantou Customs District Technology Center,Shantou 515000,Guangdong,China;Gansu International Travel Healthcare Center,Lanzhou 730020,Gansu,China;Chongqing International Travel Healthcare Center,Chongqing 401120,China;Capital Medical University,Beijing 100069,China)

机构地区：[1]中国海关科学技术研究中心,北京100026 [2]天津国际旅行卫生保健中心(天津海关口岸门诊部),天津300456 [3]汕头海关技术中心,广东汕头515000 [4]甘肃国际旅行卫生保健中心(兰州海关口岸门诊部),兰州730020 [5]重庆国际旅行卫生保健中心,重庆401120 [6]首都医科大学,北京100069

出　　处：《寄生虫与医学昆虫学报》2024年第4期245-250,共6页Acta Parasitologica et Medica Entomologica Sinica

摘　　要：目的了解“一带一路”沿线重点口岸鼠类及其携带钩端螺旋体的情况,为制定针对性病原防控有效措施提供明确的传染病分布流行依据。方法2022年9月,在10个边境口岸采用夹日法、夹夜法、笼捕法捕鼠,采集鼠类脏器和血液样本,使用梯度PCR法检测钩端螺旋体sec Y基因,对阳性扩增产物进行测序和遗传进化分析。结果从10个口岸不同生境中共捕获鼠类356只,经形态学鉴定,共计2目5科15属20种;检测到致病性钩端螺旋体核酸阳性共计7例,阳性率为1.97%(7/356);同源性对比和系统进化分析显示,北方口岸4例均为博氏钩端螺旋体Leptospira borgpetersenii,南方口岸3例均为问号钩端螺旋体Leptospira interrogans。结论“一带一路”沿线不同口岸鼠类种群构成和携带的钩端螺旋体基因型均不同,应加强口岸鼠类及其携带钩端螺旋体的监测和防控工作。Objective The study aims to understand Leptospira infection in rodents at key ports along the Belt and Road.Methods Rodents were caught at 10 border ports using night trap methods and tissues or blood samples were collected to detect the infection of Leptospira by gradient PCR and subsequent sequencing.Results A total of 356 rodents were captured from 10 ports.Morphological identification revealed 2 orders,5 families,15 genera,and 20 species.The positive rate for Leptospira infection was 1.97%by nucleic acid testing.Among them,4 cases were Leptospira borgpetersenii in the northern ports,and the rest 3 cases were L.interrogans in the southern ones.Conclusions With the different composition of rodents at different key ports along the Belt and Road,the bacteria in rodents,especially for Leptospira,differ between different port areas,suggesting the urgent need for monitoring,prevention,and control.

关 键 词：一带一路 口岸 鼠类 钩端螺旋体 

分 类 号：R377.5[医药卫生—病原生物学]