基于磷脂双层的纳米粒子微传感分析系统  

Nanoparticles microsensing and analysis system based on phospholipid bilayer

作　　者：祝可伊 薛茜男 ZHU Keyi;XUE Qiannan(College of Precision Instrument and Optoelectronics Engineering,Tianjin University,Tianjin 300072,China)

机构地区：[1]天津大学精密仪器与光电子工程学院,天津300072

出　　处：《传感器与微系统》2025年第2期62-66,共5页Transducer and Microsystem Technologies

基　　金：国家重点研发计划资助项目(2021YFC3002202);国家自然科学基金资助项目(U2233206)。

摘　　要：大气污染日益严重,悬浮纳米粒子和病毒颗粒通过肺泡进入体内,给人体带来未知的健康危害。本文通过将磷脂双层集成在场效应晶体管延长栅极表面体外模拟局部肺泡膜,设计并搭建了肺泡膜-纳米粒子相互作用实时监测系统。通过对粒子作用下源漏电流尖峰信号的检测与分析,该系统可以获取纳米粒子/病毒颗粒与肺泡膜相互作用行为的精确定量数据。测试结果表明,所提出微传感系统表面磷脂膜具有良好的完整性和生物活性,集成磷脂双层的延长栅极性能稳定、响应快、灵敏度高,可以准确、及时捕获带电离子跨膜转移信号,有望成为膜-粒相互作用行为有效的体外研究平台,推广用于广泛评估纳米粒子对人体健康的影响。Air pollution is becoming increasingly serious,suspended nanoparticles and viral particles enter human body through alveoli,bringing unknown health hazards to the human body.A real-time monitoring system of alveolar membrane-nanoparticle interaction is designed and constructed to simulate local alveolar membrane in vitro by integrating phospholipid bilayer to the extend-gate electrode of the field-effect transistor.By detecting and analyzing the peak signal of source leakage current under the action of nanoparticles,the system obtains accurate and quantitative data of the interaction behavior of nanoparticles/virus particles with alveolar membrane.tests results show that the integrated lipid membrane of the proposed microsensing system has good integrity and biological activity,the extended-gate electrode of integrated lipid bilayer show stable performance,fast response,high sensitivity,and can accurately capture the charged ion transmembrane transfer signals in real-time.The proposed system is expected to be promoted as an effective platform in vitro for the study of membrane-particle interaction behavior,evaluating the effects of nanoparticles on human health widely.

关 键 词：生物传感微系统 纳米粒子 病毒颗粒 磷脂双层 场效应管型传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]