菊花用药规律分析及其核心药物组合协同药理活性研究  

Exploring the medication rule of Chrysanthemum morifolium Ramat.and investigating the synergistic pharmacological activity of core combination herbs

作　　者：孙京甜 刘峰 张喆[1] 张玉福 马鑫慧 李庆军 王晓 董红敬[1,2,3] SUN Jingtian;LIU Feng;ZHANG Zhe;ZHANG Yufu;MA Xinhui;LI Qingjun;WANG Xiao;DONG Hongjing(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355;Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province,Shandong Analysis and Test Center,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China;Key Laboratory for Natural Active Pharmaceutical Constituents Research in Universities of Shandong Province,College of Pharmacy,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250014,China;Shandong Boda Medical Products Co.,Ltd.,Heze 274300,China)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]齐鲁工业大学(山东省科学院)山东省分析测试中心山东省大型精密分析仪器应用技术重点实验室,山东济南250014 [3]齐鲁工业大学(山东省科学院)药学院山东省高等学校天然药物活性成分研究重点实验室,山东济南250014 [4]山东博达医疗用品股份有限公司,山东菏泽274300

出　　处：《山东科学》2025年第1期1-8,共8页Shandong Science

基　　金：山东省重点研发计划(2021CXGC010508);齐鲁工业大学(山东省科学院)重大新专项(2022JBZ02-04);山东省泰山学者项目(tstp20221138);山东省重点扶持区域引进急需紧缺人才项目。

摘　　要：为分析含菊花组方的用药规律及其核心组合药物的药理活性,对含菊花方剂进行用药频次、复杂网络、关联规则分析,采用网络药理学方法构建核心组合药物的“中药-关键靶点-疾病”网络。利用预测结果,采用体外细胞模型评价菊花药物组合的药理作用。共得到含菊花组方92首,涉及药物212味,使用频次≥10的中药32味;关联规则显示菊花-甘草组合的支持度和置信度最高,其次依次为菊花-甘草-荆芥、菊花-甘草-荆芥-川芎、菊花-甘草-荆芥-川芎-防风组合。网络药理学分析显示,菊花-甘草组合用药可治疗肿瘤、消化系统疾病、神经系统疾病等。体外细胞活性研究显示,菊花-甘草药物组合对一氧化氮的抑制率优于单味药物。与菊花单味药物相比,组合用药活性更为显著,体现了中医临床用药配伍的科学性。To explore the medication rule and pharmacological activity of the core combination of chrysanthemi flos,the drug frequency,complex network and association rules of Chrysanthemum prescriptions were analyzed.The network pharmacology research method was used to construct the"herbs-key target-disease"of core combination drugs.Combined with the forecast results,an in vitro cell model was used to evaluate the pharmacological effects of chrysanthemi flos drug combinations.A total of 92 prescriptions containing chrysanthemi flos were obtained,involving 212 herbs,and 32 herbs with frequency greater than or equal to 10.The association rules showed that the sustain and confidence degree of chrysanthemi flos-Glycyrrhizae radix et rhizoma were the highest,and chrysanthemi flos-glycyrrhizae radix et rhizoma-schizonepetae herba,chrysanthemi flos-glycyrrhizae radix et rhizoma-schizonepetae herba-chuanxiong rhizoma,chrysanthemi flos-glycyrrhizae radix et rhizome-schizonepetae herba-chuanxiong rhizome-saposhnikoviae radix were next to each other,respectively.Network pharmacology analysis showed that the core drug combination of chrysanthemi flos-glycyrrhizae radix et rhizoma could treat tumors,digestive system diseases,nervous system diseases and other diseases.In vitro cell activity study showed that the combination of chrysanthemi flos and glycyrrhizae radix et rhizoma had a better inhibition rate on NO levels than the single drug.Compared with the single use of chrysanthemi flos,the combination of drugs showed more significantactivity,reflecting the scientificity of compatibility of TCM in clinic.

关 键 词：菊花 数据挖掘 用药规律 网络药理学 活性验证 

分 类 号：R285[医药卫生—中药学]