半夏泻心汤含药血清对胃癌细胞增殖影响的时效及量效关系研究  

Time-effect and dose-effect relationship study on the effect of Banxiaxiexin Decoction-containing serum on the proliferation of gastric cancer cells

作　　者：祖国秀 刘西建 祖秀丽 汤继芹[3] 黄海量[3] 韩涛 ZU Guoxiu;LIU Xijian;ZU Xiuli;TANG Jiqin;HUANG Hailiang;HAN Tao(Department of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Department of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan,250355,China;Department of Rehabilitation Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东中医药大学中医学院,山东济南250355 [2]山东中医药大学药学院,山东济南250355 [3]山东中医药大学康复医学院,山东济南250355

出　　处：《山东科学》2025年第1期9-22,共14页Shandong Science

基　　金：山东省重点研发计划(2016CYJS08A01-6);山东中医药大学首批科研创新优秀团队(220316);国家中医药管理局高水平中医药重点学科中医基础理论学科;山东中医药大学中医药经典理论教育部重点实验室。

摘　　要：采用中药血清药理学方法,观察半夏泻心汤(Banxiaxiexin Decoction,BXD)含药血清对不同胃癌细胞体外增殖的影响,并探讨用于胃癌细胞药效观察的BXD含药血清制备条件。将BXD高、中、低灌胃后30、60、90、120、150 min采血,制备含药血清。根据灌胃剂量和采血时间对MGC803、MKN45、AGS和HGC27细胞分别设5%~50%含药血清组,采用CCK8检测不同灌胃剂量、不同采血时间、不同体积分数的BXD含药血清对胃癌细胞抑制增殖的作用。实验结果显示,与其他时间点相比,BXD含药血清均在采血时间120 min时对胃癌细胞的抑制增殖作用最强;与BXD低剂量组相比,BXD中剂量组对胃癌细胞的抑制增殖作用强,且与BXD高剂量组无显著性差异。BXD中剂量组灌胃120 min后采血的含药血清按照5%~50%的10个浓度梯度,分析对不同胃癌细胞的抑制作用其IC 50值均为20%。不同灌胃剂量、不同采血时间和不同体积分数的含药血清对不同胃癌细胞的抑制作用,均显示BXD中剂量组,采血时间120 min和20%的体积分数对胃癌细胞的抑制作用最强。由此,BXD中剂量组灌胃,采血时间120 min,体积分数为20%的含药血清对胃癌细胞的药效作用最好。The pharmacological method of traditional Chinese medicine serum was used to explore the effect of Banxiaxiexin Decoction(BXD)on the proliferation of different gastric cancer cells in vitro.Additionally,the preparation conditions of BXD drug-containing serums were examined toobserve the pharmacodynamics of gastric cancer cells.Blood was collected at intervals of 30,60,90,120,and 150 minutes after high,medium,and low intragastric gavage of BXD to prepare drug-containing serums.Based on the selected gavage doses and blood collection times,MGC803,MKN45,AGS,and HGC27 cells were exposed to 5%~50%concentrations of drug-containing serum,and CCK8 assay was employed to detect the inhibitory effects of different gavage doses,blood collection times,and volume fractions of BXD drug-containing serum on the proliferation of gastric cancer cells.The test results showed that compared with other time points,all BXD drug-containing serums had the strongest inhibitory effect on gastric cancer cells at the blood collection time of 120 minutes.Compared with the low-dose BXD group,the medium-dose BXD group had a strong inhibitory effect on gastric cancer cells,and there was no significant difference between the medium-dose and high-dose BXD groups.Based on the analysis of the inhibitory effects of 10 concentrations ranging from 5%to 50%on different gastric cancer cells,the IC 50 values of all drug-containing serums collected 120 minutes after gavage in the medium-dose BXD group were 20%.Analysis of the inhibitory effects of different gavage doses,blood collection times,and volume fractions on different gastric cancer cells revealed that a medium dose of BXD,blood collection time of 120 minutes,and a volume fraction of 20%had the strongest inhibitory effect on gastric cancer cells.Therefore,the medicated serum with a blood collection time of 120 minutes and a volume fraction of 20%in the medium-dose BXD group had the best pharmacodynamic effect on gastric cancer cells.

关 键 词：半夏泻心汤含药血清 胃癌 细胞增殖 中药血清药理学 时效 量效 

分 类 号：R285[医药卫生—中药学]