D101型大孔树脂吸附纯化车前草中熊果酸的研究  

Study on Adsorption and Purification of Ursolic Acid from Plantago Asiatica L.by D101 Macroporous Resin

作　　者：陆海丽 廖慧 梁景文 LU Haili;LIAO Hui;LIANG Jingwen(Shenzhen Care-green Agriculture Products Testing&Certification Co.,Ltd.,Shenzhen 518000,China;Public Service Office of Nanwan Street,Longgang District,Shenzhen City,Shenzhen 518000,China)

机构地区：[1]深圳凯吉星农产品检测认证有限公司,广东深圳518000 [2]深圳市龙岗区南湾街道公共服务办公室,广东深圳518000

出　　处：《山东化工》2025年第1期39-42,共4页Shandong Chemical Industry

摘　　要：目的:研究从车前草提取液中富集纯化熊果酸的工艺。方法:车前草脱脂后超声提取液经酸碱沉淀初纯化,再用大孔吸附树脂进一步富集纯化熊果酸。结果:D101型大孔树脂富集纯化熊果酸的最优条件为采用质量浓度为0.7 mg/mL的车前草样品液上柱,调节pH值=7,再以90%的乙醇作为洗脱剂进行洗脱;洗脱液浓缩后经测定与熊果酸标准品的紫外出峰位置一致,纯度达79.59%。结论:该方法简便可行,为车前草熊果酸的提取及纯化提供了良好的理论和实验依据。Objective:To study the process of enrichment and purification of ursolic acid from Plantago asiatica L.extract.Methods:After defatting,the ultrasonic extract of Plantago asiatica L.was purified by acid-base precipitation,and then further enriched and purified ursolic acid by macroporous adsorption resin.Results:The optimal conditions for enrichment and purification of ursolic acid by D101 macroporous resin were to use 0.7 mg/mL of Plantago asiatica L.sample solution on the upper column,adjust pH value was 7,and then eluate with 90%ethanol as eluent.The concentration of eluent was determined to be consistent with the UV peak position of ursolic acid standard,and the purity reached 79.59%.Conclusion:This method is simple and feasible,and provides a good theoretical and experimental basis for the extraction and purification of ursolic acid from Plantago asiatica L.

关 键 词：车前草 熊果酸 大孔树脂 富集纯化 

分 类 号：TQ424[化学工程]