大鼠和兔骨髓间充质干细胞诱导分化为软骨细胞的比较  

Comparison of Chondrocytes Induced by Bone Marrow Mesenchymal Stem Cell between Rats and Rabbits

作　　者：何凤琴 任佳琪 崔雨馨 魏靖雯 林诗颖 杨晶 苗雨欣 郭美灵 HE Fengqin;REN Jiaqi;CUI Yuxin;WEI Jingwen;LIN Shiying;YANG Jing;MIAO Yuxin;GUO Meiling(School of Biological and Environmental Engineering,Xi’an University,Xi’an 710065,China;Xi’an Qinling Key Laboratory for Natural Product Research and Development and Research on Innovative Anticancer Drugs,Xi’an 710065,China)

机构地区：[1]西安文理学学院生物与环境工程学院,西安710065 [2]西安市秦岭天然产物开发与抗癌类创新药物研究重点实验室,西安710065

出　　处：《西安文理学院学报(自然科学版)》2024年第4期67-73,共7页Journal of Xi’an University(Natural Science Edition)

基　　金：国家自然科学基金项目(32270530):社会击败影响棕色田鼠社会奖赏行为的性别差异及催产素调控机制;西安市科技局农业技术攻关一般项目(23NYGG0033):乳球微粒法制备禽流感病毒抗原检测试剂盒的研发与应用;西安文理学院科研成果转化教学案例项目(JY2024AL05):医学免疫学课程教学与科研转化实践与应用;陕西省创新训练项目(DC2024044):社会击败影响C57BL/6小鼠社会奖赏行为的性别差异及多巴胺调控机制;陕西省创新训练项目(DC2024147):奶牛金黄色葡萄球菌(Staphylococcus aureus)单克隆抗体及抗原检测试剂盒的研发与应用

摘　　要：通过对大鼠和兔骨髓间充质干细胞向软骨细胞诱导分化比较研究,获得较理想软骨干细胞.分别培养大鼠和兔双侧股骨的骨髓间充质干细胞,在地塞米松,转化生长因子β_(1)蛋白(TGF-β_(1)),胰岛素等诱导因子的作用下,进行诱导培养,经甲苯胺蓝染色鉴定和Ⅱ型胶原蛋白免疫鉴定,大鼠骨髓间充质干细胞的诱导组与对照组具有极显著差异;兔骨髓间充质干细胞的诱导组与对照组具有显著性差异.大鼠的骨髓间充质干细胞中的形态已发生明显的变化,由原来的纤维状变为多角形.兔的骨髓间充质干细胞形态未发生明显变化,细胞呈聚集状态.大鼠骨髓间充质干细胞诱导分化为软骨细胞的能力更强,是较理想的干细胞.The ideal cartilage stem cells were obtained by comparing the induced differentiation of bone marrow mesenchymal stem cells(BMSCs)into chondrocytes between rats and rabbits.BMSCs from both femurs of rats and rabbits were cultured and induced by dexamethasone,transforming growth factorβ1 protein(TGF-β_(1))and insulin.And they were identified by toluidine blue staining and typeⅡcollagen immunostaining.There was a extremely significant difference in rats’BMSCs between the induction group and the control group,while there was a significant difference in rabbits’BMSCs between the induction group and the control group.The morphology of the BMSCs in rats has changed from fibrous to polygonal,while the morphology of BMSCs in rabbits did not change significantly and the cells were aggregated.The ability of rat BMSCs to differentiate into chondrocytes is more obvious,showed that rat BMSCs is an ideal stem cell.

关 键 词：骨髓间充质干细胞 诱导分化 甲苯胺蓝 Ⅱ型胶原蛋白 转化生长因子β_(1)蛋白(TGF-β_(1)) 

分 类 号：Q819[生物学—生物工程]