新疆维吾尔自治区塔额垦区腹泻羔羊粪源大肠杆菌脂多糖核心型和致病性分析  

Analysis of Fecal Source Escherichia Coli Lipopolysaccharide Core Type and Pathogenicity in Diarrhoeic Lambs from Ta′e Reclamation Area,Xinjiang Uygur Autonomous Region

作　　者：杨彬 王继学 邢国锋 赵耀 吴自豪 吴静[1] YANG Bin;WANG Jixue;XING Guofeng;ZHAO Yao;WU Zihao;WU Jing(College of Animal Sciences and Technology,Tarim University,Engineering Laboratory for Tarim Animal Diseases Diagnosis and Control of Xinjiang Production and Construction Corps,Alar843300,China;Tiemenguan Vocational and Technical College,Tiemenguan841007,China)

机构地区：[1]塔里木大学动物科学与技术学院/新疆生产建设兵团塔里木动物疫病诊断与防控工程实验室,新疆阿拉尔843300 [2]铁门关职业技术学院,新疆铁门关841007

出　　处：《畜牧与饲料科学》2024年第6期122-128,共7页Animal Husbandry and Feed Science

基　　金：新疆生产建设兵团塔里木动物疫病诊断与防控工程实验室开放课题(ELDC202002)。

摘　　要：[目的]了解新疆维吾尔自治区塔额垦区腹泻羔羊粪源大肠杆菌LPS核心型、毒力基因和致病性。[方法]从塔额垦区采集415份羔羊腹泻样本,使用鉴别培养基分离大肠杆菌,通过大肠杆菌特异性基因phoA扩增和16S rDNA序列分析对分离菌株进行分子生物学鉴定;利用PCR技术确定分离株LPS核心型和毒力基因携带情况;采用Fisher′s exact test方法分析分离株LPS核心型与毒力基因之间的相关性;通过小鼠感染试验确定分离株的致病性。[结果]从415份样本中分离、鉴定到73株大肠杆菌,分离率为17.6%(73/415);73株大肠杆菌LPS核心型主要为R1型(57.53%),其次为R3型(24.66%),R4型(10.96%)、K12型(5.48%)、R2型(1.37%)均有检出;在检测的25种毒力基因中共检测出13种毒力基因,其中,mat (98.63%)、yijP (91.78%)、ibeB (90.41%)等基因的检出率较高,irp2(16.44%)、iucD(9.59%)、stx1(2.74%)基因的检出率较低;LPS核心型与毒力基因无明显相关关系;随机选取的9株菌株可导致小鼠腹泻和死亡,对小鼠具有较强的致病性。[结论]塔额垦区腹泻羔羊粪源大肠杆菌LPS分型多样,携带多种毒力基因,具有一定的致病性,在生产中应当予以重视。[Objective]The aim of this study was to understand the core type,virulence genes and pathogenicity of Escherichia coli LPS of faecal origin in diarrhoeic lambs in Ta′e Reclamation Area,Xinjiang Uygur Autonomous Region.[Method]Four hundred and fifteen samples of diarrhoeic lambs were collected from Ta′e Reclamation Area,followed by isolation of Escherichia coli using identification medium.Then,the isolates were identified molecularly by amplification of the specific gene phoA of E.coli and 16S rDNA sequence analysis.PCR was used to determine the LPS core type and virulence gene carriage of the isolates.The correlation between LPS core type and virulence gene of the isolates was analyzed by Fisher′s exact test.The pathogenicity of the isolates was determined by mouse infection test.[Result]Seventy-three E.coli strains were isolated and identified from 415 samples with an isolation rate of 17.6%(73/415).The Seventy-three E.coli LPS core types were mainly R1(57.53%),followed by R3(24.66%),R4(10.96%),K12(5.48%)and R2(1.37%).A total of 13 virulence genes were detected among the 25 virulence genes tested,with higher detection rates for genes such as mat(98.63%),yijP(91.78%),and ibeB(90.41%),and lower detection rates for genes irp2(16.44%),iucD(9.59%),and stx1(2.74%).No significant correlation was found between LPS core type and virulence genes.In addition,nine randomly selected strains were found to cause diarrhea and death in mice and were highly pathogenic to mice.[Conclusion]The above results indicate that LPS of E.coli from lambs in the Ta′e Reclamation Area is characterized by diverse phenotypes,carries various virulence genes,and has a certain pathogenicity,which should be considered in the production.

关 键 词：羔羊 大肠杆菌 LPS核心型 毒力基因 致病性 

分 类 号：S852.612[农业科学—基础兽医学] S858.26[农业科学—兽医学]