辣木果急性毒性及亚慢性毒性研究  

The study of acute toxicity and subchronic toxicity of Moringa oleifera

作　　者：李淑琴[1] 边林秀[1] 李学敏[1] 张颖 王晓瑞[1] LI Shu-qin;BIAN Lin-xiu;LI Xue-min;ZHANG Ying;WANG Xiao-rui(Department of Toxicology,Shanxi Center for Disease Control and Prevention,Taiyuan,Shanxi 030012,China)

机构地区：[1]山西省疾病预防控制中心毒理科,山西太原030012

出　　处：《毒理学杂志》2024年第6期455-460,共6页Journal of Toxicology

摘　　要：目的研究辣木果作为新食品原料开发的食用安全性。方法20只健康SPF级昆明种小鼠,雌雄各半,采用急性经口毒性试验中的限量法,辣木果灌胃剂量为10 g/kg·bw。80只健康SPF级SD大鼠,雌雄各半,按体重随机分为4组进行亚慢性毒性试验,分别为3个剂量组和1个对照组,低、中、高3个组辣木果喂饲剂量分别为1.60、3.58和8.00 g/kg·bw,连续喂养90 d。结果辣木果对雌雄小鼠经口LD_(50)均大于10 g/kg·bw,亚慢性毒性结果显示辣木果对实验大鼠的活动、采食情况、体重增加、血液学指标、血液生化指标、脏器湿重及脏体比均未产生不良影响,各主要脏器大体解剖观察及组织显微镜检验均未见与辣木果相关的特异性病理改变。结论辣木果急性经口毒性属实际无毒级物质,亚急性毒性对大鼠的最大无作用剂量(NOAEL)为7.97 g/kg·bw,相当于每人每日推荐使用量为4.78 g,为其作为新食品原料研究提供了毒理学依据。Objective To study the edible safety of moringa oleifera as a new food raw material.Methods A total of 20 healthy SPF Kunming mice,male and female,were selected in acute oral toxicity test.The dose was 10 g/kg·bw.In subchronic toxicity test,80 healthy SPF SD rats,half male and half female,were randomly divided into four groups according to body weight,namely three dose groups and one control group,at low,medium and high doses of 1.60,3.58 and 8.00 g/kg·bw,respectively,and fed continuously for 90 days.Results The oral LD_(50)of moringa oleifonica in both male and female mice was greater than 10 g/kg·bw.The subchronic toxicity result showed that moringa oleifonica oleifonica had no adverse effects on the activity,feeding status,body mass increase,hematology index,blood biochemical index,organ wet weight and visceral-body ratio of the experimental rats.No specific pathological changes related to moringa oleifera were observed in the gross anatomy and histological examination of the major organs.Conclusion The acute oral toxicity of moringa oleifera belongs to the actual non-toxic grade.The no-observed effect level(NOAEL)toxicity in rats is 7.97 g/kg·bw,which is equivalent to the recommended daily dose of 4.78 g per person,which provides the toxicological basis for the study of moringa oleifera as a new food raw material.

关 键 词：辣木果 新食品原料 急性毒性 亚慢性毒性 实验动物 

分 类 号：R114[医药卫生—卫生毒理学] R99[医药卫生—公共卫生与预防医学]