80份香稻材料BADH2香味基因变异类型分析及稻瘟病抗性基因检测  

Analysis of Variation Types of BADH2 Aroma Genes and Detection of Rice Blast Resistance Genes in 80 Aromatic Rice Materials

作　　者：李虎[1] 吴子帅 刘广林[1] 陈传华[1] 罗群昌[1] 朱其南 LI Hu;WU Zishuai;LIU Guanglin;CHEN Chuanhua;LUO Qunchang;ZHU Qinan(Rice Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi Key Laboratory of Rice Genetics and Breeding,Nanning 530007,China)

机构地区：[1]广西农业科学院水稻研究所/广西水稻遗传育种重点实验室,南宁530007

出　　处：《中国稻米》2025年第1期35-43,53,共10页China Rice

基　　金：国家现代农业产业技术体系广西创新团队项目(nycytxgxcxtd-2021-01-03);广西农业科学院科技发展基金稳定资助科研团队项目(桂农科2021YT029);广西重点研发计划项目(桂科AB21238007,桂科AB23075129)。

摘　　要：应用目前已经开发的6个香味基因变异类型功能标记及5个稻瘟病广谱抗性基因功能标记检测80份香稻材料的Badh2香味基因变异类型及稻瘟病抗性基因,同时统计供试材料中的审定品种在本省水稻区试中稻瘟病鉴定结果,并鉴定了香味及稻瘟病表型。结果表明,80份香稻材料均具有香味,其中第4~第5外显子803 bp缺失突变类型7份(占比8.75%),同时含有5'UTR区3 bp缺失及第7外显子8 bp缺失和3 bp突变2种变异类型材料4份(占比5.00%),其他69份为第7外显子8 bp缺失和3 bp突变的变异类型(占比86.25%)。80份香稻材料中含Pita、Pi2、Pi9、Pigm、Pi1抗性基因的材料分别为57份(占比71.25%)、12份(占比15.00%)、4份(占比5.00%)、1份(占比1.25%)和1份(占比1.25%);同时含Pi2、Pita的材料有9份(占比11.25%),同时含Pigm、Pita的材料1份(占比1.25%),同时含Pi1、Pita的材料1份(占比1.25%)。同时携带2个抗性基因的材料表现抗病的比例高于只携带1个抗性基因及不携带抗性基因的材料。研究结果为香稻材料的应用及抗稻瘟病基因聚合、分子标记辅助育种提供依据。Using six aroma gene variation types and five rice blast broad-spectrum resistance gene functional markers,we detected the Badh2 aroma gene variant types and rice blast resistance genes in 80 aromatic rice materials.At the same time,we also counted the rice blast identification results of the approved varieties in rice regional trial,and identified the aroma and rice blast phenotype.The results showed that all 80 aromatic rice materials possessed aroma,with 7 samples(accounting for 8.75%)having a 803 bp deletion mutation in exons 4 to 5,and 4 samples(accounting for 5.00%)containing two types of variants with a 3 bp deletion in the 5’UTR region and an 8 bp deletion and 3 bp mutation in exon 7.The remaining 69 samples(accounting for 86.25%)had the variant type of 8 bp deletion and 3 bp mutation in exon 7.Among the 80 aromatic rice materials,the numbers containing the Pita,Pi2,Pi9,Pigm,and Pi1 resistance genes were 57(71.25%),12(15.00%),4(5.00%),1(1.25%),and 1(1.25%),respectively.There are 9 materials containing both Pi2 and Pita(accounting for 11.25%),1 material containing both Pigm and Pita(accounting for 1.25%),and 1 material containing both Pi1 and Pita(accounting for 1.25%).The proportion of materials carrying two resistance genes simultaneously exhibiting disease resistance is higher than that of materials carrying only one resistance gene and those without resistance genes.The research results provide a basis for the application of aromatic rice materials and the pyramiding of rice blast resistance genes,as well as for molecular marker-assisted breeding of aromatic rice.

关 键 词：香稻 香味基因 稻瘟病抗性基因 

分 类 号：S511.035[农业科学—作物学]