衰老相关分泌表型在冠状动脉粥样硬化患者血清中的表达及与血管狭窄的关系  

Expression of aging related secretory phenotype in serum of patients with coronary atherosclerosis and its relationship with vascular stenosis

作　　者：罗丹 成娜娜 徐锡浩 王珺楠[1] LUO Dan;CHENG Nana;XU Xihao;WANG Junnan(Department of Cardiology,the Second Hospital of Jilin University,Changchun 130022,China;ChinaIntensive Care Unit,the First Hospital of Jilin University,Changchun 130021,China)

机构地区：[1]吉林大学第二医院心血管内科,吉林长春130022 [2]吉林大学第一医院重症医学科,吉林长春130021

出　　处：《中国实验诊断学》2025年第1期19-23,共5页Chinese Journal of Laboratory Diagnosis

摘　　要：目的分析衰老相关分泌表型(SASP)在冠状动脉粥样硬化患者血清中的表达变化并评估其能否作为预测血管狭窄程度的生物学标志物。方法选取自2023年3月至2023年7月进行冠状动脉造影(CAG)的105名患者,根据CAG结果将其分为冠状动脉狭窄组和对照组。比较两组患者血清SASP(IL-6、TNF-α、CXCL-1及MMP-2)的水平;分析SASP与冠状动脉狭窄程度的相关性;采用受试者工作特征(ROC)曲线探究血清SASP对冠状动脉狭窄程度的诊断价值。结果两组患者在年龄、体质指数(BMI)、性别、高血压、糖尿病、吸烟史、饮酒史等基线数据以及总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)等实验室指标差异均没有统计学意义(P>0.05),但冠状动脉狭窄组患者血清中IL-6、TNF-α、CXCL-1及MMP-2水平高于对照组,差异有统计学意义(P<0.05);多因素Logistic回归分析表明IL-6、TNF-α、CXCL-1及MMP-2是冠状动脉狭窄的危险因素;Spearman相关分析提示IL-6、TNF-α、CXCL-1及MMP-2水平与冠状动脉狭窄程度呈正相关(P<0.05);最后通过ROC曲线评估SASP对冠状动脉狭窄程度的诊断价值,4项指标联合诊断的ROC曲线下面积(AUC)为0.937,显著高于IL-6、TNF-α、CXCL-1及MMP-2单独检测的AUC值(分别为0.805、0.855、0.713和0.828)。结论SASP水平在冠状动脉狭窄程度高的患者血清中显著升高,可作为冠脉血管狭窄的潜在预测指标。Objective The aim of this study is to analyze the expression of senescence associated secretory phenotype(SASP)in the serum of patients with coronary atherosclerosis and evaluate whether it can be used as the biomarker to predict the degree of vascular stenosis.Methods Select 105patients who underwent coronary angiography(CAG)from March 2023to July 2023.Divide them into the coronary artery stenosis group and the control group.Compare the levels of SASP(IL-6,TNF-α,CXCL-1and MMP-2)in serum between two groups of patients.Analyze the correlation between the levels of SASP with the degree of coronary artery stenosis.Assess the diagnostic value of serum SASP for coronary artery stenosis using receiver operating characteristic(ROC)curve analysis.Results There was no significant difference between the two groups in age,BMI,sex,hypertension,diabetes,smoking history,drinking history,as well as laboratory indicators such as total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C)(P>0.05).But the serum levels of IL-6,TNF-α,CXCL-1and MMP-2in patients with coronary artery stenosis were higher than those in the control group,with statistically significant differences(P<0.05).Multivariate logistic regression analysis showed that IL-6,TNF-α,CXCL-1and MMP-2were independent risk factors for coronary artery stenosis.Spearman correlation analysis suggests that the levels of IL-6,TNF-α,CXCL-1and MMP-2are positively correlated with the degree of coronary artery stenosis,and the difference was statistically significant(P<0.05).Finally,the area under the ROC curve(AUC)for the combined diagnosis of the four indicators was 0.937,which was significantly higher than the AUC values of IL-6,TNF-α,CXCL-1and MMP-2detected separately(0.805、0.855、0.713and 0.828respectively).Conclusion The level of SASP is significantly elevated in the serum of patients with high degree of coronary artery stenosis,and can serve as a potential predictor of coronary artery stenosis.

关 键 词：冠状动脉粥样硬化 衰老相关分泌表型 白介素-6 肿瘤坏死因子-α 趋化因子-1 金属蛋白酶-2 

分 类 号：R54[医药卫生—心血管疾病]