加味射干麻黄汤调节磷脂酰肌醇3激酶/蛋白激酶B信号通路干预寒性哮喘大鼠气道重塑机制研究  

Effects of the Shegan Mahuang decoction on airway remodeling in cold asthma rats by regulating PI3K/Akt signaling pathway

作　　者：耿志霞 刘聪[2] 张爱利 倪艳[2] GENG Zhixia

机构地区：[1]山西中医药大学附属医院,山西太原030024 [2]山西省中医药研究院中药方剂研究所,山西太原030012

出　　处：《中医临床研究》2024年第34期1-7,共7页Clinical Journal Of Chinese Medicine

基　　金：山西中医药大学附属医院青年基金培训项目(QN202112)。

摘　　要：目的:研究加味射干麻黄汤对卵清蛋白(Ovalbumin,OVA)雾化联合冷刺激诱导的寒性哮喘大鼠的治疗作用及气道重塑机制。方法:将60只雄性大鼠随机分为空白组、模型组、醋酸泼尼松片组(2.5 mg/kg)和加味射干麻黄汤高剂量组(22.95 g/kg)、加味射干麻黄汤中剂量组(11.4 g/kg)、加味射干麻黄汤低剂量组(5.74 g/kg)。除空白组外,其余组别大鼠腹腔注射10%的OVA溶液1 mL,并联合冷刺激建立大鼠寒性哮喘模型。将模型大鼠灌胃给予相应药物,空白组与模型组给予等体积的生理盐水,连续15天,给药前后观察、记录大鼠体质量及一般状态。采用酶联免疫吸附试验(Enzyme-linked Immunosorbent Method,ELISA)检测大鼠血清中白细胞介素(Interleukin,IL)-1β、IL-6含量。采用苏木精-伊红(HE)染色,观察大鼠肺组织病理学改变;免疫组化法检测大鼠肺组织磷脂酰肌醇3激酶(Phosphatidylinositol3-Kinase,PI3K)、凋亡信号调节激酶1(Apoptosis Signal Regulating Kinase 1,ASK1)的表达丰度。结果:体质量及一般状态:空白组大鼠毛色整洁、光亮,活动自如,体质量稳定增长,未见异常;模型组及各给药组大鼠皮毛散乱无华、呼吸急促、明显腹部收缩,咳嗽、畏寒、大便溏;加味射干麻黄汤各剂量组大鼠摄食量、饮水量及寒性哮喘症状明显改善,体质量与空白组相比未见差异(P> 0.05)。血清IL-1β、IL-6含量:与空白组相比,模型组IL-1β、IL-6含量显著升高(P <0.05或P <0.01);加味射干麻黄汤高剂量组、加味射干麻黄汤中剂量组IL-1β、IL-6含量有所下降(P <0.05或P <0.01)。肺组织病理学观察:空白组肺组织支气管结构无明显变异,结构完整,无炎性细胞浸润;模型组大鼠支气管结构发生改变,可见多种炎性细胞浸润、黏液栓及气管平滑肌增生,而加味射干麻黄汤各剂量组均有不同程度的改善。免疫组化法检测肺组织PI3K、ASK1表达丰度:与正常组比较,模型组PI3K、ASK1Objective:To study the therapeutic effect of the Shegan Mahuang decoction(加味射干麻黄汤)on cold asthma induced by OVA atomization combined with cold stimulation and the mechanism of airway remodeling.Methods:A total of 60 male rats were randomly divided into blank group,model group,prednisone acetate tablet group(2.5 mg/kg),the Shegan Mahuang decoction high-dose,medium-dose and low-dose groups(22.95,11.48,5.74 g/kg).In addition to the blank group,the other groups of rats were intraperitoneally injected with 10%OVA solution 1 mL and combined with cold stimulation to establish a rat model of cold asthma.The rats model were given corresponding drugs by intragastric administration,and the blank group and model group were given equal volume of normal saline for 15 consecutive days.The weight and general state of the rats were observed and recorded before and after administration.The contents of interleukin-1β(IL-1β)and interleukin-6(IL-6)in serum of rats were detected by ELISA.HE staining was used,and histopathological changes of lung were observed.The expression abundance of PI3K and ASK1 in lung tissues of rats was detected by immunohistochemistry.Results:Body weight and general status:In the blank group,the fur color of rats was clean and bright,the rats were self-active,and the body weight was increased steadily without abnormality.The rats in the model group and administration groups had disorderly hair,shortness of breath,obvious abdominal contraction,cough,chills and loose stool.After administration,food intake,water intake and symptoms of cold asthma of rats in the Shegan Mahuang decoction groups were significantly improved,and there was no difference in body weight compared with the blank group(P>0.05).Serum IL-1βand IL-6 contents:Compared with the blank group,the contents of IL-1βand IL-6 in the model group were significantly increased(P<0.05 or P<0.01).After administration,the contents of IL-1βand IL-6 in the Shegan Mahuang decoction high-dose and middle-dose groups were decreased(P<0.05 or P<0

关 键 词：加味射干麻黄汤 磷脂酰肌醇3激酶 凋亡信号调节激酶1 哮喘 气道重塑 

分 类 号：R256.12[医药卫生—中医内科学]