接种植物根际促生菌对施用有机无机复混肥土壤N_(2)O排放的影响  

Effects of inoculation with plant growth-promoting rhizobacteria on N_(2)O emission from soil applied with organic and inorganic compound fertilizer

作　　者：王洲章 朱津宏 熊若男 杨思琪 高南 吴永红[3] 张振超[4] 吴国平[4] 申卫收 WANG Zhou-zhang;ZHU Jin-hong;XIONG Ruo-nan;YANG Si-qi;GAO Nan;WU Yong-hong;ZHANG Zhen-chao;WU Guo-ping;SHEN Wei-shou(School of Environmental Science and Engineering,Nanjing University of Information Science and Technology/Jiangsu Provincial Key Laboratory of Atmospheric Environment Monitoring and Pollution Control/Jiangsu Provincial Collaborative Innovation Center of Atmospheric Environment and Equipment Technology,Nanjing Jiangsu 210044;College of Biological and Pharmaceutical Engineering,Nanjing University of Technology/National Research Center for Biochemical Engineering Technology,Nanjing Jiangsu 211816;Institute of Soil Science,Chinese Academy of Sciences,State Key Laboratory of Soil and Sustainable Agricultural Development,Nanjing Jiangsu 210008;Zhenjiang Institute of Agricultural Sciences,Hilly Region of Jiangsu,Zhenjiang Jiangsu 212400)

机构地区：[1]南京信息工程大学环境科学与工程学院/江苏省大气环境监测与污染控制高技术研究重点实验室/江苏省大气环境与装备技术协同创新中心,江苏南京210044 [2]南京工业大学生物与制药工程学院/国家生化工程技术研究中心,江苏南京211816 [3]中国科学院南京土壤研究所,土壤与农业可持续发展国家重点实验室,江苏南京210008 [4]江苏丘陵地区镇江农业科学研究所,江苏镇江212400

出　　处：《中国土壤与肥料》2024年第12期42-51,共10页Soil and Fertilizer Sciences in China

基　　金：国家自然科学基金(31972503,41771291);江苏省重点研发计划(社会发展)项目(BE2020731)。

摘　　要：施用有机无机复混肥可提高土壤肥力、增加作物产量,但也增加温室气体氧化亚氮(N_(2)O)的排放。采集江苏省句容市的复垦土壤样品设置室内土壤培养试验,研究接种植物根际促生菌(PGPR)对施用有机无机复混肥土壤N_(2)O排放的影响。结果表明,接种PGPR菌株Azospirillum sp.TSA2S、Pseudomonas stutzeri NRCB010、Achromobacter denitrificans YSQ030和Bacillus subtilis NRCB002,相对于施肥不接种的对照显著减少了土壤N_(2)O排放量;土壤N_(2)O的累积排放量由高到低为NRCB002>TSA2S>NRCB010>YSQ030,其中N_(2)O排放量分别减少了59%、62%、63%和72%。无论接种与否,施肥均显著提高了土壤中氨氧化古菌amoA、氨氧化细菌amoA以及全程硝化菌amoB、反硝化细菌nirS和nirK的基因拷贝数。接种携带nosZ基因的N_(2)O还原细菌A.sp.TSA2S、P.stutzeri NRCB010、A.denitrificans YSQ030显著提高了施肥土壤中nosZⅠ和nosZⅡ的基因丰度。然而,接种不携带nosZ基因的B.subtilis NRCB002则没有明显改变施肥土壤中nosZⅠ和nosZⅡ的基因丰度。这些结果意味着接种4株具有N_(2)O减排效应的PGPR菌株,分别从微生物介导的直接和间接机制减少了土壤N_(2)O的排放。研究结果将为减少施用有机无机复混肥土壤N_(2)O排放提供科学依据,也将为研发具有生态环境效应的新型生物肥料提供技术参考。The application of organic and inorganic compound fertilizers can improve soil fertility and crop yield,but it also increases nitrous oxide(N_(2)O)emission.In this study,reclaimed soil from Jurong city,Jiangsu province was collected for laboratory soil incubation experiment.The effects of inoculation with plant growth-promoting rhizobacteria(PGPR)on the N_(2)O emission from soil applied with the organic and inorganic compound fertilizer were investigated.The results showed that the inoculation with PGPR strains Azospirillum sp.TSA2S,Pseudomonas stutzeri NRCB010,Achromobacter denitrificans YSQ030,and Bacillus subtilis NRCB002 significantly decreased cumulative N_(2)O emissions from soil compared with the fertilization and non-inoculated control.The cumulative N_(2)O emissions were as follows:NRCB002>TSA2S>NRCB010>YSQ030,which were decreased by 59%,62%,63%and 72%,respectively,after their inoculation in comparison with the non-inoculated control.The gene copy numbers of archaeal and bacterial amoA,amoB,and denitrifier nirS and nirK in soil were significantly increased after the fertilization,irrespective of inoculation with these strains or not.The inoculation with N_(2)O-reducing denitrifier A.sp.TSA2S,P.stutzeri NRCB010,and A.denitrificans YSQ030,which possess nosZ gene,significantly increased the gene copy numbers of nosZⅠand nosZⅡin soil.However,the inoculation with B.subtilis NRCB002 without nosZ gene,did not change the gene copy numbers of nosZⅠand nosZⅡ.These results suggested that these four PGPR strains decreased soil N_(2)O emissions possibly through direct and indirect microbial-mediated mechanisms,respectively.The study will provide scientific basis and practical guidance for mitigating N_(2)O emission from soil with organic and inorganic compound fertilizer application,and will also provide technical reference for developing novel biofertilizer with ecological and environmental effects.

关 键 词：复垦土壤 植物根际促生菌(PGPR) 氧化亚氮(N_(2)O) 有机无机复混肥 氮循环功能基因 

分 类 号：S14[农业科学—肥料学]