外源茉莉酸甲酯对镉胁迫下大豆种子萌发及幼苗生理的影响  

Effects of exogenous methyl jasmonate on soybean seed germination and physiological characteristics under cadmium stress

作　　者：崔晓霞 石晨乐 刘学勤 CUI Xiao-xia;SHI Chen-le;LIU Xue-qin(College of Agronomy and Horticulture,Jangsu Vocational College of Agriculture and Forestry,Jurong Jiangsu 212400)

机构地区：[1]江苏农林职业技术学院农学园艺学院,江苏句容212400

出　　处：《中国土壤与肥料》2024年第12期204-210,共7页Soil and Fertilizer Sciences in China

基　　金：国家自然科学基金项目(32301776);江苏农林职业技术学院院级科技项目(2020kj007)。

摘　　要：以大豆‘Williams 82’为试验材料,分析不同浓度的外源茉莉酸甲酯(MeJA)溶液对重金属镉胁迫下的大豆种子萌发、幼苗生长过程中生理指标的影响,探寻缓解大豆镉胁迫的最适合MeJA浓度。试验采用不同浓度的外源MeJA溶液对重金属镉胁迫下的大豆种子和幼苗分别进行处理,结果显示,大豆种子的萌发及幼苗生理指标在镉离子浓度为0.1 mmol/L时受到了明显的抑制,外源施加较低浓度的MeJA可以有效促进大豆种子的萌发,降低大豆幼苗叶片中游离脯氨酸和丙二醛的含量,超氧化物歧化酶、过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶的活性则相应提高;外源施加大于150μmol/L浓度的MeJA则表现出对种子萌发和活性氧代谢的抑制作用,结果说明适宜浓度的外源MeJA可以有效地缓解重金属镉胁迫对大豆幼苗的损害,而太高浓度的MeJA则会对大豆幼苗的生长和代谢产生不利影响。因此,100μmol/L的外源MeJA可最有效地促进镉胁迫下大豆种子的萌发和幼苗生长。To investigate the effects of exogenous methyl jasmonate(MeJA)on seed germination and seedling physiological characteristics of soybean under cadmium stress,different concentrations of exogenous MeJA were used to treat the seeds and seedlings of soybean under cadmium stress.The germination and physiological index of soybean were studied.The result showed that the germination of soybean seeds and physiological indicators of seedlings were significantly inhibited at a cadmium concentration of 0.1 mmol/L.Application of lower concentrations of exogenous MeJA effectively promoted the germination of soybean seeds,reduced the content of free proline and malondialdehyde in soybean seedling leaves,and increased the activities of superoxide dismutase,peroxidase,catalase and ascorbate peroxidase.Application of exogenous MeJA at high concentrations greater than 150μmol/L actually exhibited inhibitory effects on seed germination and reactive oxygen species metabolism.The results indicated that appropriate concentrations of exogenous MeJA effectively alleviated the damage of heavy metal cadmium stress to soybean seedlings,while excessively high concentrations of MeJA had adverse effects on the growth and metabolism of soybean seedlings.Therefore,100μmol/L of exogenous MeJA could effectively promote the germination and seedling growth of soybean seeds under cadmium stress.

关 键 词：大豆 镉胁迫 茉莉酸甲酯 萌发 生理特性 

分 类 号：Q94[生物学—植物学]