盐蒿根际产ACC脱氨酶菌株的筛选及其促生效果研究  

Screening of ACC-deaminase strains from the rhizosphere of Artemisia halodendron and study on their growth-promoting effect

作　　者：黄欣 陈燕 谷青青 徐秋霞 吴玲 王莹[2] 郭聪 曹凡 李玉娟[2] HUANG Xin;CHEN Yan;GU Qing-qing;XU Qiu-xia;WU Ling;WANG Ying;GUO Cong;CAO Fan;LI Yu-juan(Institute of Agricultural Resources and Environment,Jiangsu Academy of Agricultural Sciences,Nanjing Jiangsu 210014;Jiangsu Yanjiang Institute of Agricultural Sciences,Nantong Jiangsu 226002)

机构地区：[1]江苏省农业科学院农业资源与环境研究所,江苏南京210014 [2]江苏沿江地区农业科学研究所,江苏南通226002

出　　处：《中国土壤与肥料》2024年第12期223-229,共7页Soil and Fertilizer Sciences in China

基　　金：江苏省农业科技自主创新资金项目(CX233133);南通市社会民生科技计划项目(MS22022059);南通市社会民生科技计划项目(MS2023056);江苏沿江地区农业科学研究所青年科技基金(YJ2022004)。

摘　　要：为了获取植物促生菌资源,提高植物耐盐胁迫能力,从盐蒿根际筛选产1-氨基环丙烷-1-羧酸(ACC)脱氨酶耐盐菌株,通过分子生物学手段进行鉴定并通过发芽试验和盆栽试验验证促生效果。从盐蒿根际筛选出两株产ACC脱氨酶耐盐菌株,其中菌株AhR1的ACC脱氨酶酶活为15.72μmol/(mg·h),菌株AhR5的ACC脱氨酶酶活为48.38μmol/(mg·h),除此之外两株菌还具有产生物被膜、产吲哚乙酸、解无机磷等功能。经鉴定,菌株AhR1和AhR5均属于假单胞菌属(Pseudomonas)。油菜种子发芽试验表明,两株菌的低浓度菌液(OD_(600)=0.1)均能够显著促进2‰~6‰盐含量下油菜根和芽的生长。油菜盆栽试验表明,菌株AhR1在4‰盐含量下对油菜干重的提升最大,达到22.07%;菌株AhR5在2‰盐含量下对油菜干重的提升最大,达到29.91%。菌株AhR1和AhR5均能够明显促进盐胁迫下油菜的生长,为盐碱地微生物肥料的开发和土壤改良提供了菌种资源和理论支持。To acquire resources for promoting plant growth and enhancing plant tolerance to salt stress,the salt-tolerant strains which could produce 1-aminocyclopmpane-1-carboxylate(ACC)-deaminase from the rhizosphere of Artemisia halodendron were screened and identified by molecular biological methods.The growth-promoting effect was confirmed through germination tests and pot experiments.Two salt-tolerant strains producing ACC-deaminase were screened.Strains AhR1 and AhR5,identified as belonging to Pseudomonas,exhibited ACC-deaminase activity of 15.72 and 48.38μmol/(mg·h),respectively.Additionally,both strains were found to produce biofilm,indoleacetic acid,and decompose inorganic phosphorus.The results indicated that at salt concentrations of 2‰-6‰,the low concentration(OD_(600)=0.1)of the two strains significantly promoted the growth of rapeseed roots and buds.The results of the pot experiment on rapeseed indicated that strain AhR1 increased the dry weight of rapeseed by 22.07%at 4‰salt content,while strain AhR5 increased it by 29.91%at 2‰salt content.These findings suggested that strains AhR1 and AhR5 significantly promoted the growth of rapeseed under salt stress,and provided valuable resources for the development of microbial fertilizers and soil improvement in saline-alkali land.

关 键 词：盐蒿 根际促生菌 ACC脱氨酶 

分 类 号：S43[农业科学—农业昆虫与害虫防治]