蒲公英总黄酮通过调控肠道菌群对小鼠肥胖的抑制作用机制  

Mechanism of inhibitory effect of total flavonoids from Taraxacum mongolicum on obesity in mice by regulating intestinal flora

作　　者：郜怡雪 郭琳 郎林艳[1] 吴静 王浩阳 杨静 苗明三[2,3] 李占占[2,3] GAO Yixue;GUO Lin;LANG Linyan;WU Jing;WANG Haoyang;YANG Jing;MIAO Mingsan;LI Zhanzhan(College of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Academy of Chinese Medical Science,Henan University of Chinese Medicine,Zhengzhou 450046,China;Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,郑州450046 [2]河南中医药大学中医药科学院,郑州450046 [3]豫药全产业链研发河南省协同创新中心,郑州450046

出　　处：《中国药房》2025年第3期293-299,共7页China Pharmacy

基　　金：国家自然科学基金项目(No.82074038,No.82304831);国家中医药管理局科技项目(No.GZYKJS-2022-040-1);河南省自然科学基金“优秀青年科学基金”项目(No.242300421090)。

摘　　要：目的 探讨蒲公英总黄酮通过调控肠道菌群对高脂饮食诱导的小鼠肥胖的抑制作用机制。方法 将24只C57BL/6J小鼠随机分为空白组、模型组和蒲公英总黄酮组,每组8只。除空白组外,其余两组小鼠予以高脂饮食饲养,同时蒲公英总黄酮组小鼠予蒲公英总黄酮[400 mg/(kg·d)]灌胃,每天1次,连续8周。实验期间,记录每组小鼠的采食量。末次给药后,对小鼠的体重、脂肪质量、血脂水平及肝脏和附睾脂肪病理变化进行评价,观察蒲公英总黄酮对小鼠肥胖的治疗效果;通过扩增子测序检测小鼠肠道菌群丰度及结构的变化;利用实时荧光定量PCR技术分析蒲公英总黄酮对小鼠脂肪代谢相关基因的影响。结果 与模型组比较,蒲公英总黄酮组小鼠的体重显著降低(P<0.05);血清总胆固醇、甘油三酯、低密度脂蛋白胆固醇水平均显著降低(P<0.01),高密度脂蛋白胆固醇水平显著升高(P<0.01);腹股沟白色脂肪组织和附睾白色脂肪组织的脂肪指数均显著降低(P<0.05);肝细胞脂肪变性及脂肪细胞病变明显改善;细胞色素c氧化酶亚基7A1和细胞色素c氧化酶亚基8B mRNA的表达均显著上调(P<0.05)。菌群检测结果显示,与模型组比较,蒲公英总黄酮组小鼠的菌群多样性有上升趋势,Sobs指数和β多样性均显著升高(P<0.05);经黏液真杆菌属Blautia、norank_f_Ruminococcaceae、嗜胆菌属Bilophila、另枝菌属Alistipes、classi-fied_f_Ruminococcaceae、副拟杆菌属Parabacteroides、norank_f_Desulfovibrionaceae、厌氧短杆菌属Anaerotruncus的菌群相对丰度显著升高(P<0.05),粪杆菌属Faecalibaculum、丹毒梭菌属Erysipelatoclostridium、GCA-900066575、梭菌属Tuzzerella、乳杆菌属Lactobacillus、norank_f_norank_o_RF39、achnospiraceae_FCS020_group的菌群相对丰度显著下降(P<0.05)。结论 蒲公英总黄酮能降低肥胖小鼠的体重、脂肪质量与血脂水平,修复肥胖小鼠肝脏及附睾脂肪的病理损伤,这�OBJECTIVE To investigate the mechanism of the inhibitory effect of total flavonoids from Taraxacum mongolicum on high-fat diet-induced obesity in mice through modulation of intestinal flora.METHODS Twenty-four C57BL/6J mice were randomly divided into blank group,model group and T.mongolicum total flavonoid group,with 8 mice in each group.Except for the blank group,the other 2 groups were given a high-fat diet,while T.mongolicum total flavonoid group was given T.mongolicum total flavonoid[400 mg/(kg·d)]intragastrically,once a day,for 8 consecutive weeks.During the experiment,the food intake of each group of mice was recorded.After the last medication,the body mass,fat weight,blood lipid level and pathological changes of liver and epididymal fat in mice were evaluated to observe the effect of T.mongolicum total flavonoid on the treatment of obesity in mice.The changes in abundance and structure of intestinal flora in mice were detected by amplicon sequencing;the effects of T.mongolicum total flavonoids on fat metabolism related genes were analyzed by qPCR.RESULTS Compared with model group,the body weight of mice in T.mongolicum total flavonoids group was decreased significantly(P<0.05);the levels of total lipid cholesterol,triglycerides,and LDL cholesterol were all decreased significantly(P<0.01),and the level of HDL cholesterol was increased significantly(P<0.01);the fat indexes of inguinal white adipose tissue and epididymal white adipose tissue were significantly reduced(P<0.05);significant improvement in hepatocellular steatosis and adipose cytopathy were significantly improved;mRNA expressions of COX7A1 and COX8B were significantly upregulated(P<0.05).The results of bacterial colony detection showed that compared with the model group,there was a rising trend in the diversity of the bacterial colony in T.mongolicum total flavonoids group,and the Sobs index characterization andβdiversity were increased significantly(P<0.05).Relative abundances of Blautia,norank_f_Ruminococcaceae,Bilophila,Alistipes,classified_

关 键 词：蒲公英总黄酮 肥胖 肠道菌群 脂肪代谢 

分 类 号：R965[医药卫生—药理学]