Integrated analysisof monocyte infiltration and differential gene expressionin hypertrophic obstructive cardiomyopathy  

作　　者：QIN Xian-yu TAN Jian ZHENG Hao-sheng ZHENG Yu-zhen LIAO Hong-ying ZHUANG Jian 

机构地区：[1]Department of Thoracic Surgery,Thoracic Cancer Center,The Sixth Affiliated Hospital,Sun Yat-sen University,Guangzhou 510655,Guangdong,China [2]Biomedical Innovation Center,The Sixth Affiliated Hospital,Sun Yat-sen University,Guangzhou 510655,China [3]Guangdong Cardiovascular Institute,Guangdong Provincial Key Laboratory of South China Structural Heart Disease,Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences,Guangzhou 510100,Guangdong Province,China

出　　处：《South China Journal of Cardiology》2024年第4期260-274,共15页岭南心血管病杂志(英文版)

基　　金：National Natural Science Foundation of China(No.82102955);Guangzhou Basic Research Project(No.02201011326)。

摘　　要：Background Hypertrophic obstructive cardiomyopathy(HOCM)is one of the main reasons for sudden cardiac death(SCD)in young people.Researches has revealed that immune-related genes are closely relevant to HOCMprogression.Therefore,it is important to explore the key immuneregulatory mechanisms and biomarkersof HOCM progression.Methods The bioinformatics methods,including linear models for microarray analysis(LIMMA),protein-protein interaction(PPI)network,Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes pathway(KEGG)and CIBERSORT,were used to assess the key pathways and hub genes involved in HOCM.Furthermore,expression levels of hub genes were validatedin human tissue.Results Our results showed that the degree of infiltration of five immune cells was linked to HOCM progression,including monocytes,macrophages M2,natural killer(NK)cell resting,B cells native,and T cells regulatory(Tregs).A total of 7 hub genes(CCL2,CXCL8,FOS,MAP2K1,NFKBIA,STAT3,and TNFRSF1A)were identified and validated by quantitative real-time polymerase chain reaction(qt-PCR).The core genes including CCL2,MAP2K1,NFKBIA,STAT3,and TNFRSF1A are closely related to monocytes infiltration during HOCM progression.Conclusions Taken together,our research provided useful information to explore the immune mechanisms underlying HCM progression and to provide a potential therapeutic target for therapy in HOCM.The interactional relationship of GATA5,BCL3,and ATF1 complex-regulation CCL2,MAP2K1,NFKBIA,STAT3,and TNFRSF1A was involved in the regulation of monocytes tissue infiltration,which was closely related to the progression of HOCM.

关 键 词：Hypertrophic cardiomyopathy Hypertrophic obstructive cardiomyopathy Immune infiltration Immune cell subtype 

分 类 号：R54[医药卫生—心血管疾病]