青枯菌侵染下不同类型烟草根系转录组比较分析  

Comparative Analysis of Root Transcriptomes from Different Types of Tobacco Cultivars under Ralstonia solanacearum Infection

作　　者：章慧芬 张玉芹 曹长代 孙刚[4] 温亮 刘洋[4] 刘正文 肖志亮 耿锐梅[1] 任民[1] 李丽华[6] 杨爱国[1] ZHANG Huifen;ZHANG Yuqin;CAO Changdai;SUN Gang;WEN Liang;LIU Yang;LIU Zhengwen;XIAO Zhiliang;GENG Ruimei;REN Min;LI Lihua;YANG Aiguo(Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Le’an Branch of Fuzhou Tobacco Company of Jiangxi Province,Fuzhou 344300,Jiangxi,China;Shandong Rizhao Tobacco Co.,Ltd.,Rizhao 276826,Shandong,China;Shandong Weifang Tobacco Co.,Ltd.,Weifang 261205,Shandong,China;Shandong Linyi Tobacco Co.,Ltd.,Linyi 276003,Shandong,China;Luoyang Branch of Henan Tobacco Company,Luoyang 471000,Henan,China)

机构地区：[1]中国农业科学院烟草研究所,青岛266101 [2]江西省抚州市乐安县烟草分公司,江西抚州344300 [3]山东日照烟草有限公司,山东日照276826 [4]山东潍坊烟草有限公司,山东潍坊261205 [5]山东临沂烟草有限公司,山东临沂276003 [6]河南省烟草公司洛阳市公司,河南洛阳471000

出　　处：《中国烟草科学》2024年第6期7-16,共10页Chinese Tobacco Science

基　　金：中国烟草总公司重大科技项目[110202201008(JY-08)];中国烟草总公司山东省公司重点项目(202303);中国农业科学院科技创新工程(ASTIP-TRIC01)。

摘　　要：采用转录组测序(RNA-seq)方法,探究了雪茄烟品种Beinhart 1000-1与烤烟品种小黄金1025在青枯菌侵染下的转录响应差异。结果显示,在侵染I阶段,Beinhart 1000-1中鉴定到568个优势表达基因,主要富集在碳水化合物代谢与淀粉和蔗糖代谢等通路,小黄金1025中鉴定到1028个优势表达基因,主要富集在信号转导与转录因子等通路;结合全基因组家族成员鉴定,在Beinhart 1000-1优势表达基因中鉴定到15个细胞壁合成与重塑相关基因以及3个信号转导相关的APETALA2/ethylene responsive factor(AP2/ERF)家族成员,而小黄金1025优势表达基因中分别鉴定到3和24个上述相关成员;此外,侵染I阶段Beinhart 1000-1根系中的青枯菌生物量明显少于小黄金1025。可见,Beinhart 1000-1与小黄金1025在青枯菌侵染早期阶段转录响应存在较大差异。RNA sequencing was used to investigate the differentially expressed genes between cigar tobacco Beinhart 1000-1 and flue-cured tobacco Xiaohuangjin1025 under Ralstonia solanacearum infection for clarifying the transcriptomic differences.The results showed that 568 preferentially expressed genes were identified in Beinhart 1000-1 at the infection stage I and enriched in carbohydrate metabolism and starch and sucrose metabolism pathways,while 1028 preferentially expressed genes were identified in Xiaohuangjin1025 and enriched in signal transduction and transcription factor pathways.Furthermore,15 genes related to cell wall biosynthesis and remodeling and 3 members of AP2/ERF gene family were detected in the preferentially expressed genes of Beinhart 1000-1,while 3 and 24 genes were identified,respectively,in Xiaohuangjin1025.Besides,the biomass of Ralstonia solanacearum in roots of Beinhart 1000-1 was much smaller than that in Xiaohuangjin1025.In all,this study showed that there were distinct differences between Beinhart 1000-1 and Xiaohuangjin1025 in early transcriptional response to Ralstonia solanacearum infection.

关 键 词：烟草 青枯病 转录组 优势表达基因 抗性机制 

分 类 号：S572.03[农业科学—烟草工业]