HPLC法同时测定四味脾胃舒制剂中3种成分的含量  

Simultaneous Determination of Three Components in Siwei Piweishu Preparations by HPLC

作　　者：张远芳 刘芳 何艳红 姚秋凤 周丽倩 黄璐 Zhang Yuanfang;Liu Fang;He Yanhong;Yao Qiufeng;Zhou Liqian;Huang Lu(School of Pharmacy,Xiangnan University,Chenzhou 423000,China)

机构地区：[1]湘南学院药学院,湖南郴州423000

出　　处：《中国野生植物资源》2024年第12期58-62,共5页Chinese Wild Plant Resources

基　　金：湖南省自然科学基金项目(2021JJ40520);2023年国家级大学生创新训练计划项目(S202310545012);2023年湖南省大学生创新创业训练计划项目(湘教通[2023]237号);2022年湘南学院实验室开放项目(校发[2023]18号);湖南省药学应用特色学科资金资助[湘教通(2018)469号];2024年湖南省教育厅科学研究项目(湘教通[2024]251号)。

摘　　要：目的:建立高效液相色谱法(HPLC)同时测定四味脾胃舒颗粒、四味脾胃舒片中鸡屎藤苷酸、鸡屎藤苷酸甲酯和橙皮苷的含量测定方法,并进行成分含量测定。方法:采用InertSustain AQ-C_(18)色谱柱(4.6 mm×250 mm,5μm),检测波长为240 nm,流动相为乙腈(A)-0.1%磷酸水溶液(B)梯度洗脱,柱温为30℃,流速为1.0 mL/min,进样量为10μL。结果:鸡屎藤苷酸线性回归方程为Y=9461.7X+45330(r≥0.9995),鸡屎藤苷酸甲酯线性回归方程为Y=9360.4X+444.74(r≥0.9999),橙皮苷线性回归方程为Y=4674.6X-11081(r≥0.9997)。颗粒中鸡屎藤苷酸、鸡屎藤苷酸甲酯和橙皮苷的每日服用量范围分别为17.10~48.90 mg/d、2.70~7.50 mg/d、18.30~24.00 mg/d。片剂中鸡屎藤苷酸、鸡屎藤苷酸甲酯和橙皮苷每日服用量范围分别为13.08~19.02 mg/d、1.14~4.02 mg/d、15.06~22.02 mg/d。结论:该方法适用于四味脾胃舒颗粒、四味脾胃舒片的质量控制。Objective:A high performance liquid chromatography(HPLC)method was established for the simultaneous determination of paederosidie acid,paederosidic acid methyl ester,and hesperidin in Siwei Piweishu Granules and Siwei Piweishu Tablets.Methods:The InertSustain AQ-C_(18) chromatographic column(4.6 mm×250 mm,5μm)was used.The detection wavelength was 240 nm.The mobile phase consisted of acetonitrile(A)and 0.1% phosphoric acid aqueous solution(B)for gradient elution.The column temperature was maintained at 30℃,the flow rate was 1.0 mL/min,and the injection volume was 10μL.Results:The linear regression equation for paederosidie acid was Y=9461.7X+45330(r≥0.9995),the linear regression equation for paederosidic acid methyl ester was Y=9360.4X+444.74(r≥0.9999),and the linear regression equation for hesperidin was Y=4674.6X-11081(r≥0.9997).For the granules,the daily intake ranges of paederosidie acid,paederosidic acid methyl ester,and hesperidin were 17.10-48.90 mg/d,2.70-7.50 mg/d,and 18.30-24.00 mg/d,respectively.For the tablets,the daily intake ranges of paederosidie acid,paederosidic acid methyl ester,and hesperidin were 13.08-19.02 mg/d,1.14-4.02 mg/d,and 15.06-22.02 mg/d,respectively.Conclusion:The method employed in this study could be utilized for the quality control of Siwei Piweishu Granules and Siwei Piweishu Tablets.

关 键 词：四味脾胃舒颗粒 四味脾胃舒片 含量测定 

分 类 号：R927.2[医药卫生—药学]