大气CO_(2)浓度升高球孢白僵菌定殖对玉米植株生长及光合的影响  

Effects of Beauveria bassiana colonization on the growth and photosynthesis of maize plants under elevated CO_(2)

作　　者：文欢 张正坤[1] 李启云[1,2,3] 路杨 隋丽[1] WEN Huan;ZHANG Zhengkun;LI Qiyun;LU Yang;SUI Li(Jilin Key Laboratory of Agricultural Microbiology,Key Laboratory of Integrated Pest Management on Crops in Northeast China,Ministry of Agriculture and Rural Areas,Institute of Plant Protection,Jilin Academy of Agricultural Science,Gongzhuling 136100,China;College of Plant Protection,Jilin Agricultural University,Changchun 130118,China;Jilin Agricultural Science and Technology University,Jilin 132101,China)

机构地区：[1]吉林省农业科学院植物保护研究所,吉林省农业微生物重点实验室,农业农村部东北作物有害生物综合治理重点实验室,公主岭136100 [2]吉林农业大学植物保护学院,长春130118 [3]吉林农业科技学院,吉林132101

出　　处：《生态学报》2025年第2期974-983,共10页Acta Ecologica Sinica

基　　金：吉林省农业科技创新工程项目(CXGC2024JJ011);国家自然科学基金项目(32271683)。

摘　　要：球孢白僵菌(Beauveria bassiana)能够在植物内定殖并促进植物的生长,同时能够提高植物抗胁迫能力。全球大气CO_(2)浓度不断升高,CO_(2)浓度升高对植物生长具有重要影响,同时对植物-微生物互作也会产生调控作用。然而,CO_(2)浓度升高条件下球孢白僵菌定殖对植物生长发育的影响及其作用机制鲜有研究。为明确CO_(2)浓度升高条件下球孢白僵菌定殖对植物生长的影响及作用机理,采用盆栽控制试验,在开顶式气室内研究CO_(2)浓度升高条件下球孢白僵菌定殖对玉米植株生长参数和光合特性的作用。结果表明,采用灌根法能够使球孢白僵菌成功定殖于玉米植株中,对玉米生长有正向调控作用。在CO_(2)浓度增加条件下经球孢白僵菌处理的玉米植株,全生育期内玉米植株高度、叶面积、生物量和百粒重均有所提高。此外,利用激光共聚焦显微镜观察结果显示,各处理组间玉米叶片气孔数量和形态大小变化情况均存在一定差异,在玉米各生育期内,球孢白僵菌处理组气孔数量比对照组显著降低,在CO_(2)浓度升高条件下接种球孢白僵菌,气孔数量显著增加,同时,在CO_(2)浓度升高条件下接种球孢白僵菌,气孔长度和气孔宽度在各生育期均降低,其中球孢白僵菌处理组气孔长度和宽度最小。CO_(2)浓度升高和球孢白僵菌定殖对玉米光合指标影响显著,其中净光合速率和胞间CO_(2)浓度显著升高,蒸腾速率和气孔导度均降低。研究结果表明,CO_(2)浓度升高和球孢白僵菌定殖能够通过增强植物的光合作用促进植物生长。研究解析了CO_(2)浓度增加条件下球孢白僵菌对玉米植株生长的影响及其作用机理,为明确球孢白僵菌定殖促进玉米生长发育和提高玉米产量提供科学依据,为进一步指导气候变化背景下虫生真菌的应用提供技术支撑和理论指导。Beauveria bassiana can colonize and promote the growth of many plants,especially improving the biotic/abiotic stress resistance of host plants.Elevated CO_(2)is one of the key environmental drivers with far-reaching to plant growth,and it also has a regulatory effect on plant-microbial interaction.However,little is known about the effects and mechanisms of B.bassiana on plant growth and development under elevated CO_(2)concentration.To clarify the effects and mechanisms of B.bassiana colonization on plant growth under increased CO_(2)concentration,maize and B.bassiana were studied in this study as important species in agroecosystem,and a controlled experiment was conducted to study the effects of B.bassiana colonization on the growth and photosynthetic characteristics of maize plants under elevated CO_(2)were studied in Open-top chambers(OTCs).The results showed that the root irrigation method facilitated the colonization of B.bassiana in maize,which promoted maize growth,and the maize treated with B.bassiana demonstrated increased plant height,leaf area,biomass,and weight per 100 seeds throughout the entire growth period under elevated CO_(2)concentration.Furthermore,it was found that there were significantly differences in the number and morphological size of stomata in maize leaves among different groups under laser confocal microscopy,the number of stomata in the B.bassiana treatment was significantly lower than that in the control group,and the number of stomata was significantly increased under elevated CO_(2)concentration,at all growth stages,at the same time,the length and width of stomata decreased at each growth stage when B.bassiana was inoculated with increased CO_(2)concentration,and the length and width of stomata in B.bassiana treatment were the smallest;the increase of CO_(2)concentration and colonization of B.bassiana had significant effects on the photosynthetic indicators of maize leaves,such as net photosynthetic rate and intercellular CO_(2)concentration were significantly increased,and the t

关 键 词：气候变化 虫生真菌 内生定殖 促生作用 光合特性 

分 类 号：S513[农业科学—作物学]