定海湾龟足肠道微生物组成及代谢组分析  

Analysis on Intestinal Microbiota Composition and Metabolome of Capitulum mitella in Dinghai Bay

作　　者：赵文博 刘鑫 林岗[1,2] 姚海燕 郑泽文 ZHAO Wen-bo;LIU Xin;LIN Gang;YAO Hai-yan;ZHENG Ze-wen(College of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350117,China;Fujian Key Laboratory for the Sustainable Utilization of Special Marine Biological Resources,Fuzhou,Fujian 350117,China;Institute of Oceanography,Minjiang University,Fuzhou,Fujian 350117,China)

机构地区：[1]福建师范大学生命科学学院,福建福州350117 [2]福建省特色海洋生物资源可持续利用重点实验室,福建福州350117 [3]闽江学院海洋研究院,福建福州350117

出　　处：《福建农业科技》2024年第11期9-17,共9页Fujian Agricultural Science and Technology

基　　金：中央引导地方科技发展专项(2022L3010)。

摘　　要：为了解定海湾龟足肠道微生物群落结构特点及代谢组特征,探索龟足生存环境、食性及肠道微生物组成之间的联系,使用16S rRNA高通量测序技术和非靶向代谢组学技术,对采集的龟足样品进行分析。结果表明:龟足肠道菌群在门水平上,最优势菌门为变形菌门,含量为47.17%,其次为拟杆菌门,含量为40.61%;在纲水平上,最优势纲为拟杆菌纲,含量为40.59%,其次为γ变形菌纲,含量为32.62%;在属水平上,最优势属为Sediminibacterium,含量为22.77%,其次为Pseudarcicella,含量为10.97%,还存在较多未能培养和未能鉴定的菌属。龟足肠道代谢物中含量最多的为脂类及类脂物质,约占代谢物总量的31%,其次为有机酸及其衍生物,约占代谢物总含量的23%。In order to understand the characteristics of intestinal microbial community structure and metabolome of Capitulum mitella in Dinghai Bay,and explore the relationship between the living environment,feeding habits and intestinal microbial composition of Capitulum mitella,the collected samples of Capitulum mitella were analyzed by using the 16S rRNA high-throughput sequencing technology and non-targeted metabolomics technology.The results showed that at the phylum level,the most dominant phylum was Proteobacteria,with a content of 47.17%,followed by Bacteroidetes,with a content of 40.61%.At the class level,the most dominant class was Bacteroides,with a content of 40.59%,followed by Gammaproteobacteria,with a content of 32.62%.At the genus level,the most dominant genus was Sediminibacterium,with a content of 22.77%,followed by Pseudarcicella,with a content of 10.97%.There were still more genera that failed to be cultured or identified.The most abundant metabolites in the intestinal tract of Capitulum mitella were lipide and lipid materials,accounting for about 31% of the total metabolites,followed by organic acids and their derivatives,accounting for about 23% of the total metabolites.

关 键 词：定海湾 龟足 肠道微生物 代谢组 

分 类 号：S917[农业科学—水产科学]