白杨素对酒精性肝病模型小鼠肠道菌群的影响  

Effects of Chrysin on the Intestinal Flora in Mice with Alcoholic Liver Disease Model

作　　者：董璐 张昊天 康妍钰 王菲[1] 郭浩林 董颖 杨勇 白婷 DONG Lu;ZHANG Haotian;KANG Yanyu;WANG Fei;GUO Haolin;DONG Ying;YANG Yong;BAI Ting(Dalian Key Laboratory of Chronic Disease Research Center,Dalian University,Dalian 116622,China)

机构地区：[1]大连大学慢性病研究中心大连市重点实验室,大连116622

出　　处：《医药导报》2025年第2期176-182,共7页Herald of Medicine

基　　金：国家自然科学基金资助项目(81900532,81700523);大连大学学科交叉项目(DLUXK-2024-QN-012)。

摘　　要：目的探究白杨素对酒精性肝病(ALD)模型小鼠肠道菌群的影响。方法将小鼠随机分为正常对照组、ALD模型组、水飞蓟素组、白杨素小剂量组、白杨素大剂量组(25、50 mg·kg^(-1))。采用乙醇液体饲料喂养8周合并单剂量乙醇(5 g·kg^(-1))灌胃造模。灌胃给药8周后,收集血清,采用试剂盒检测5组小鼠血脂及肝功能指标;收集肝脏,苏木精-伊红(HE)染色观察肝脏病理变化;收集肠道内容物,采用16 S rDNA高通量测序方法,研究5组小鼠的肠道微生物整体变化。结果与ALD模型组比较,白杨素小、大剂量组天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、三酰甘油(TG)含量均明显下降,且能够缓解酒精引起的肝细胞脂肪变性和炎症反应。16S rDNA测序结果显示,ALD模型组小鼠肠道菌群总数以及种类显著减少,优势菌属改变为埃希-志贺菌Escherichia-Shigella、嗜黏蛋白阿克曼菌Akkermansia。白杨素小、大剂量组小鼠肠道菌群较ALD模型组Shannon指数明显提升;在门和属水平上,白杨素大剂量组丰度显著升高,菌群总数及数量增加且优势菌群颤螺菌属Oscillospirales、厚壁菌属g-Firmicutes、拟普雷沃菌属Alloprevotella的丰度变化尤为突出。结论白杨素可能通过改善ALD模型小鼠肠道菌群失调发挥对ALD的治疗作用。Objective To explore the effect of chrysin on intestinal flora in mice with alcoholic liver disease(ALD).Methods Mice were randomly assigned to normal control group,ALD model group,Silymarin group,chrysin low-dose group,chrysin high-dose group(25,50 mg·kg^(-1)).The mice were fed with alcoholic liquid diet and a single dose of alcohol(5 g·kg^(-1))for eight weeks to establish the ALD model.After eight weeks of oral administration,each group's serum and plasma lipids and liver function indices were collected and detected using kits;then collected the liver and observed the pathological changes of the liver using HE staining;meanwhile,intestinal contents were collected and changes in mouse gut flora were analyzed by 16S rDNA sequencing.Results Compared with the ALD group,the level of aspartate transaminase(AST),alanine transaminase(ALT)and triacylglycerol(TG)of low-dose and high-dose chrysin groups were significantly reduced,and it can alleviate liver cell steatosis and inflammatory reactions caused by alcohol.16S rDNA results showed that the total number and types of intestinal flora in the ethanol group were significantly reduced,as well as a change in the dominant genus to Escherichia-Shigella and Akkermansia.Compared to the ALD model group,the Shannon index of the intestinal microbiota increased significantly in mice treated with low and high doses of chrysin.In addition,at the phylum and genus level,the abundance of the high-dose chrysin group increased significantly,resulting in an overall increase in the total number and amount of microbiota.The abundance of dominant bacterial groups,such as Oscillospirales,irmicutes,and Alloprevotella,was also significantly increased.Conclusion Chrysin may exert therapeutic effects on ALD by improving intestinal flora imbalance in ALD mice.

关 键 词：白杨素 酒精性肝病 肠道菌群 16S rDNA基因测序 厚壁菌属 拟普雷沃菌属 

分 类 号：R563[医药卫生—呼吸系统]