基于HPLC指纹图谱与化学计量学研究常山柚橙幼果与枳实的相似性  

Similarity Between Young Fruits of Citrus aurantium‘Changshan-huyou’and Aurantii Fructus Immaturus Based on HPLC Fingerprint and Chemometrics

作　　者：王笑笑 胡卫南 王健 郑建李 邵晓锋 罗菲 WANG Xiaoxiao;HU Weinan;WANG Jian;ZHENG Jianli;SHAO Xiaofeng;LUO Fei(Quzhou Institute for Food and Drug Control,Quzhou 324002,China;Quzhou Nankong Chinese Medicine Co.Ltd,Quzhou 324002,China;Quzhou College of Technology,Quzhou 324002,China)

机构地区：[1]衢州市食品药品检验研究院,衢州324002 [2]衢州南孔中药有限公司,衢州324002 [3]衢州职业技术学院,衢州324002

出　　处：《医药导报》2025年第2期273-280,共8页Herald of Medicine

基　　金：浙江省药品监督管理局2021年度科技计划项目(2021013);浙江省“十四五”育种专项中药材协作组项目(2021C02074)。

摘　　要：目的探索常山柚橙幼果与药典基源枳实的相似性情况。方法采用高效液相色谱法建立常山柚橙幼果与枳实的指纹图谱,采用中药色谱指纹图谱相似度评价系统软件进行相似度分析,利用化学计量学对常山柚橙幼果与枳实全组分比较,并对14种化合物进行定量分析。结果①枳实间相似度与聚类分析结果受基源、产地的影响较大,甜橙与其他基源枳实的相似度仅有0.2;不同产地的酸橙相似度在0.802~0.986,相对离散;②常山柚橙幼果与部分酸橙、代代、臭橙和朱栾聚为一类,相似度>0.9;③变量重要性投影值(VIP)法筛选出21个对质量有重要作用的共有峰,主要有新橙皮苷、柚皮苷、伞形花内酯、川陈皮素、水合橙皮内酯和橙皮苷等。定量分析结果显示:常山柚橙幼果和枳实中富含柚皮苷和新橙皮苷,橙皮苷与芸香柚皮苷次之;不同基源枳实间主成分含量差异很大,特别是甜橙,常山柚橙幼果则相对稳定。结论常山柚橙幼果与大部分基源枳实具有较好的相似度,作为枳实药用具有一定的可行性。Objective To explore the similarity between young fruits of Citrus aurantium‘Changshan-huyou’and Pharmacopoeia origin Aurantii Fructus Immaturus.Methods The fingerprints of young fruits of Citrus aurantium‘Changshan-huyou’and the Pharmacopoeia origin Aurantii Fructus Immaturus were established by high performance liquid chromatography(HPLC).The similarity analysis was carried out by using the software of Traditional Chinese medicine chromatographic fingerprint similarity evaluation system.The whole components of young fruit of Citrus aurantium‘Changshan-huyou’and the Pharmacopoeia origin Aurantii Fructus Immaturus were compared by using chemometrics,and the 14 compounds were analyzed quantitatively.Results①The results of similarity and cluster analysis were affected by the origin and producing area,for example,the similarity between citrus sinensis(L.)Osbeck and other origin was only 0.2,the similarity of citrus aurantium L.from different producing areas ranged from 0.802 to 0.986,with relative large dispersion;②young fruit of Citrus aurantium‘Changshan-huyou’,some citrus aurantium L.,citrus aurantium L.cv.Daidai,stink orange and citrus aurantium L.cv.Zhulan all belong to the same category,and the similarity was above 0.9;③VIP method screened out 21 common peaks that had important effects on quality,mainly including neohesperidin,naringin,umbellitin,tangeretin,hesperidin hydrate and hesperidin.The quantitative analysis results showed that Naringin and neohesperidin were abundant in young fruits of Citrus aurantium‘Changshan-huyou’and Aurantii Fructus Immaturus,followed by hesperidin and naringin,there were significant differences in the content of principal components among Aurantii Fructus Immaturus from different origins,especially in citrus sinensis(L.)Osbeck,the young fruits of Citrus aurantium‘Changshan-huyou’was relatively stable.Conclusion The young fruits of Citrus aurantium‘Changshan-huyou’had good similarity with most origin of Aurantii Fructus Immaturus,so it is feasib

关 键 词：常山柚橙幼果 枳实 指纹图谱 高效液相色谱 化学计量学 

分 类 号：R931[医药卫生—生药学]