基于转录组测序分析附子须根成结的分子机制  

Analyzing Molecular Mechanism of Knot Formation in Fuzi Fibrous Root Based on RNA-seq

作　　者：章景 李永宁 袁鑫怡 王富华 高继海[1] 彭成[1] Zhang Jing;Li Yongning;Yuan Xinyi;Wang Fuhua;Gao Jihai;Peng Cheng(State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu,611137,China)

机构地区：[1]成都中医药大学药学院西南特色中药资源国家重点实验室,四川成都611137

出　　处：《成都中医药大学学报》2025年第1期20-27,共8页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家自然科学基金重大专项(81891010,81891012);国家中医药多学科交叉创新团队项目(ZYYCXTD-D-202209)。

摘　　要：目的:基于附子栽培基地中出现的须根成结现象,探究其形成的分子机制。方法:本研究针对附子须根成结现象,利用高通量测序技术对附子、须根及根结转录组进行分析,探究附子须根成结的分子机制。结果:本研究共获得119.15 Gb Clean Data,794466条unigenes,共有43899条unigenes在8大数据库得到注释。KEGG富集显示,共有28814条unigenes参与到138条通路中,其中植物-病原菌互作通路注释最为丰富,另有68条基因注释到参与植物防御病菌害的WRKY转录因子家族。通过差异表达基因分析,发现病原微生物通过侵害角质层、细胞壁突破植物防御,3-酮酰基-CoA合成酶和纤维素合成酶在防御过程中发挥重要作用。UTP-葡萄糖-1-磷酸尿苷酰转移酶、1,4-α-葡聚糖分支酶、蔗糖合酶均在膨大根结中表达上调,提示淀粉和蔗糖代谢通路是根结膨大的重要途径。结论:本研究解析了附子须根成结的发生发育机制,为研究附子抗病原微生物提供理论基础。Objective:To investigate the molecular mechanism of knot formation based on the phenomenon of fibrous knot formation that occurs in Fuzi cultivation sites.Methods:In this study,we analyzed the Fuzi,fibrous root and root-knot transcriptomes using high-throughput sequencing to address the phenomenon of root-knot growth on fibrous root.Results:As a result,a total of 119.15 Gb Clean Data,794466 unigenes were obtained,and a total of 43899 unigenes were annotated in 8 major databases.In the KEGG database,a total of 28814 unigenes were involved in 138 pathways,of which the plant-pathogen interaction pathway was the most abundantly annotated,and an additional 68 genes annotated to the WRKY family of transcription factors involved in plant defense against pathogen damage.Analysis of differentially expressed genes revealed that pathogenic microorganisms may break through plant defenses by attacking the cuticle and cell wall,and that 3-ketoacyl-CoA synthase and cellulose synthase play important roles in the defense process.UTP-glucose-1-phosphate uridyltransferase,1,4-α-glucan branching enzyme,and sucrose synthase were all up-regulated in expression in expanded root nodules,and it was hypothesized that starch and sucrose metabolism pathway was an important pathway for root nodule expansion.Conclusion:This finding analyzed the developmental mechanism of Fuzi root nodulation and provided a theoretical basis for studying the resistance of Fuzi to pathogenic microorganisms.

关 键 词：附子 须根 根结 转录组测序 病原微生物 

分 类 号：R285[医药卫生—中药学]