电针对2型糖尿病大鼠血清脂多糖及肠道屏障损伤的影响  

Effect of Electroacupuncture on Serum LPS and Intestinal Barrier Damage in Type 2 Diabetic Rats

作　　者：王香 张月 陈炫宜 洪肖娟[1] Wang Xiang;Zhang Yue;Chen Xuanyi;Hong Xiaojuan(Chengdu University of Traditional Chinese Medicine,Chengdu,610075,China)

机构地区：[1]成都中医药大学针灸推拿学院,四川成都611137

出　　处：《成都中医药大学学报》2025年第1期36-41,共6页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81973961);四川省科技厅基金项目(2022NSFSC0857)。

摘　　要：目的:观察电针对2型糖尿病(Type 2 Diabetes Mellitus,T2DM)大鼠血清脂多糖(Lipopolysaccharide,LPS)及肠道屏障的影响,探讨电针治疗T2DM的作用机制。方法:24只雄性SD大鼠随机分为空白组、模型组、电针组,每组8只。模型组、电针组采用高脂高糖喂养4周结合小剂量链脲佐菌素(Streptozocin,STZ)建立T2DM大鼠模型。电针组予针刺双侧“足三里”“三阴交”和“胃脘下俞”穴,双侧“足三里”“三阴交”连接电极,予连续波,频率为10 Hz,留针30 min,6次/周,干预4周。干预后,测定大鼠空腹血糖(Fasting Blood Glucose,FBG)、口服糖耐量实验(Oral Glucose Tolerance Test,OGTT),检测血清空腹胰岛素(Fasting Insulin,FINS)和脂多糖(Lipopolysaccharide,LPS)水平的变化,苏木精-伊红(Hematoxylin-eosin staining,HE)染色观察结肠组织形态学变化;实时荧光定量PCR(Real-time-polymerase Chain reaction,qRT-PCR)检测结肠组织闭锁小带蛋白-1(Zonula Occludens-1,ZO-1)、紧密连接蛋白-1(Claudin-1)、闭锁蛋白(Occludin)mRNA表达变化,Western blot(WB)法检测结肠组织ZO-1,Claudin-1,Occludin蛋白表达变化。结果:与空白组相比,模型组大鼠FBG水平显著升高(P<0.01),HE可见结肠黏膜形态破坏,肠腺排列紊乱,有明显的炎性细胞浸润,血清FINS、LPS水平升高(P<0.01),结肠组织ZO-1,Claudin-1,Occludin的mRNA和蛋白表达减少(P<0.01);与模型组相比,电针组FBG水平显著降低(P<0.01),HE染色可见电针组大鼠结肠黏膜形态相对完好,肠道黏膜结构得到改善,炎性渗出减少,血清FINS、LPS水平降低(P<0.05、P<0.01),结肠组织ZO-1、Claudin-1、Occludin mRNA和蛋白表达增加(P<0.05、P<0.01)。结论:电针能降低T2DM大鼠模型血糖、空腹胰岛素及血清LPS水平,上调肠道屏障ZO-1,Claudin-1和Occludin蛋白的表达,从而减轻T2DM的症状。Objective:To observe the effects of electroacupuncture(EA)on serum LPS and intestinal barrier in type 2 diabetes mellitus(T2DM)rats,and exploring the mechanism of EA in the treatment of T2DM.Methods:24 male SD rats were randomly divided into blank group,model group,and electroacupuncture group,with 8 rats in each group.The model group and electroacupuncture group were fed a high-fat and high-sugar diet for 4 weeks combined with a small dose of streptozocin(STZ)to establish a T2DM rat model.In the electroacupuncture group,bilateral acupoints"Zusanli"(ST36),"Sanyinjiao"(SP6),and"Weiwanxiashu"(EX-B3)were needled,with bilateral"ST36"and"SP6"connected to electrodes,using continuous waves at a frequency of 10 Hz,retaining the needles for 30 minutes,6 times per week for 4 weeks.After the intervention,fasting blood glucose(FBG)of rats was measured,an oral glucose tolerance test(OGTT)was performed,and changes in serum fasting insulin(FINS)and lipopolysaccharide(LPS)levels were detected.Colon tissue morphology was observed by HE staining;real-time fluorescence quantitative PCR(qRT-PCR)was used to detect changes in the expression of zonula occludens-1(ZO-1),claudin-1,and occludin mRNA in colon tissue;Western blot(WB)was used to detect changes in the expression of ZO-1,claudin-1,and occludin proteins in colon tissue.Results:Compared to the blank group,the model group of rats showed a significant increase in FBG levels(P<0.01).Histological examination(HE staining)revealed damage to the colonic mucosal morphology,disordered arrangement of intestinal glands,noticeable infiltration of inflammatory cells,elevated serum levels of FINS and LPS(P<0.01),and decreased expression of ZO-1,Claudin-1,and Occludin mRNA and protein in colonic tissues(P<0.01).In comparison to the model group,the electroacupuncture group showed a significant decrease in FBG levels(P<0.01).HE staining indicated relatively intact colonic mucosal morphology in rats from the electroacupuncture group,improvement in intestinal mucosal structure,reduced inflammatory

关 键 词：2型糖尿病 电针 血糖 脂多糖 肠道屏障 

分 类 号：R245[医药卫生—针灸推拿学] R256[医药卫生—中医临床基础]