十五烷酸对高脂日粮处理的妊娠期母鼠肠道形态的影响  

Effect of pentadecanoic acid on intestinal morphology in pregnant female mice treated with high-fat diets

作　　者：郑世奇 李梓莹 何衍乐 张妍璘 苟钟勇 张薇 卢浩鹏 杨梦林 王玮 黄燕华 彭杰 ZHENG Shiqi;LI Ziying;HE Yanle;ZHANG Yanlin;GOU Zhongyong;ZHANG Wei;LU Haopeng;YANG Menglin;WANG Wei;HUANG Yanhua;PENG Jie(Innovative Institute of Animal Healthy Breeding,Zhongkai University of Agriculture and ngineering,Guangzhou,Guangdong Province 510225,China;Key Laboratory of Animal Nutrition and Feed Science in South China,Ministry of Agriculture in Rural Affairs Guangdong Key Laboratory of Animal Breeding and Nutrition,Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong Province 510640,China;Guangdong Laboratory for Lingnan Modern Agricultural,Guangzhou,Guangdong Province 510640,China)

机构地区：[1]仲恺农业工程学院健康养殖创新研究院,广东广州510225 [2]广东省农业科学院动物科学研究所,广东省畜禽育种与营养研究重点实验室,农业农村部华南动物营养与饲料重点实验室,广东广州510640 [3]岭南现代农业科学与技术广东省实验室,广东广州510642

出　　处：《中国饲料》2025年第1期37-44,共8页China Feed

基　　金：国家自然科学基金(32202755);广东省普通高校重点领域专项(2022ZDZX4021);广东省基础与应用项目(2021A1515012417);广东省大学生创新创业训练计划项目(S202311347012)。

摘　　要：为探究高脂日粮(HFD)和十五烷酸(C15:0)对妊娠期母体肠道形态结构的影响,试验选取32只10周龄C57BL/6J雌性小鼠进行交配,观察到阴道栓后记为0.5 d,并将妊娠母鼠随机分为4组,每组8只,分别饲喂低脂日粮(LFD)、低脂日粮+十五烷酸(LFD+1%C15:0)、高脂日粮(HFD)、高脂日粮+十五烷酸(HFD+1%C15:0),饲养期18 d。当母鼠妊娠日龄达到18.5 d时收集母鼠十二指肠、空肠、回肠、结肠并进行HE染色,观察肠道形态结构。结果显示:饲喂HFD显著降低了十二指肠的隐窝深度,并提高了十二指肠绒隐比,添加C15:0显著提高了十二指肠上皮细胞厚度。在十二指肠形态学指标上,HFD饮食与C15:0无交互作用。饲喂HFD显著提高了空肠隐窝深度,并降低空肠绒隐比,添加C15:0对空肠形态结构无显著影响。在空肠形态学指标上,HFD饮食与C15:0无交互作用。饲喂HFD显著增加了回肠绒毛宽度和绒毛表面积,添加C15:0对其无显著影响。在回肠肠上皮细胞厚度指标上,HFD饮食与C15:0产生了交互作用,其中HFD组显著高于LFD组。饲喂HFD和添加C15:0分别对结肠形态无显著影响,但在结肠肠上皮细胞厚度上,HFD饮食和C15:0产生了交互作用,其中HFD组显著高于LFD组和HFD+1%C15:0组。综上所述,饲喂HFD未对妊娠期母体十二指肠和结肠形态结构产生不利影响,但有损害空肠和回肠形态结构趋势,而C15:0不能缓解HFD引起的空肠和回肠损伤趋势,但有改善十二指肠上皮屏障功能的作用,具有一定的应用价值。The aim of this study was to investigate the effects of high fat diet(HFD)and pentadecanoic acid(C15:0)on the intestinal morphology and structure of mammals during pregnancy.Thirty-two 10-week-old C57BL/6J female mice were selected for mating in the experiment,and the presence of a vaginal pessary was recorded as 0.5 d of pregnancy.Pregnant females were randomly divided into four groups of eight rats each,and were fed a low fat diet(LFD),low fat diet+pentadecanoic acid(LFD+1%C15:0),a high fat diet(HFD),and a high fat diet+pentadecanoic acid(HFD+1%C15:0)for a feeding period of 18 d.The duodenum,jejunum,ileum,and colon of female rats were collected and subjected to HE staining when the gestational age of female rats reached 18.5 d to observe the intestinal morphology and structure.The results showed that feeding HFD significantly reduced the depth of duodenal crypts and increased the duodenal villous-crypt ratio,and the addition of C15:0 significantly increased the thickness of duodenal epithelial cells.There was no interaction between the HFD diet and C15:0 on duodenal morphological indices.Feeding HFD significantly increased jejunal crypt depth and decreased jejunal villous crypt ratio,and the addition of C15:0 had no significant effect on jejunal morphological structure.There was no interaction between HFD diet and C15:0 on jejunal morphological indices.Feeding HFD significantly increased ileal villus width and villus surface area,which were not significantly affected by the addition of C15:0.The HFD diet interacted with C15:0 on the ileal intestinal epithelial cell thickness index,which was significantly higher in the HFD group than in the LFD group.Feeding HFD and addition of C15:0 had no significant effect on colonic morphology,respectively,but on colonic intestinal epithelial cell thickness,HFD diet and C15:0 produced an interaction,in which the HFD group was significantly higher than the LFD group and the HFD+1%C15:0 group.In conclusion,feeding HFD did not adversely affect maternal duodenal and colonic morp

关 键 词：十五烷酸 高脂 妊娠 肠道 

分 类 号：S816.7[农业科学—饲料科学]