羊栖菜水提物基于Nrf2/ARE信号通路改善HepG2细胞的氧化应激和胰岛素抵抗的作用机制研究  

Study on the Mechanism of Improving Oxidative Stress and Insulin Resistance of HepG2 Cells by Using Nrf2/ARE Signaling Pathway

作　　者：吴金华 胡兰芳 罗煌群 蒲柳艳 赵碧娜 张斌 黄烈平 WU Jinhua;HU Lanfang;LUO Huangqun;PU Liuyan;ZHAO Bina;ZHANG Bin;HUANG Lieping(Department of Anesthesiology,Zhoushan Maternal and Child Health Hospital,Zhoushan 316000,China;School of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316000,China)

机构地区：[1]舟山市妇幼保健院,浙江舟山316000 [2]浙江海洋大学食品与药学院,浙江舟山316000

出　　处：《海南师范大学学报(自然科学版)》2024年第4期475-481,共7页Journal of Hainan Normal University(Natural Science)

基　　金：舟山市科技计划项目(2023C31014,2024C31015);浙江省中医药科技计划项目(2024ZL1230);浙江海洋大学人才引进科研基金(JX6311131523)。

摘　　要：型糖尿病的主要症状包括胰岛素抵抗、高血糖和胰岛素缺乏。尽管一些口服降糖药物可以有效改善胰岛素抵抗,但其中大多数药物,包括双胍类、噻唑烷二酮类和磺脲类药物会带来严重的不良反应。这促使人们对天然来源的治疗Ⅱ型糖尿病的药物产生了兴趣。在本研究中,探讨了羊栖菜水提物基于Nrf2/ARE信号通路改善HepG2细胞的氧化应激和胰岛素抵抗的作用机制,经过不同浓度的羊栖菜水提物处理后,超氧化物歧化酶和谷胱甘肽过氧化物酶显著增加,活性氧和脂质氧化丙二醛水平也显著逆转;同时胰岛素抵抗HepG2细胞中核因子红细胞2相关因子2、kelch样环氧氯丙烷相关蛋白和血红素加氧酶1的蛋白水平显著增加;以及核因子E2相关因子2、血红素加氧酶1和胰岛素受体底物1的基因表达显著增加,进一步证实了,羊栖菜水提物对由高糖诱导的胰岛素抵抗HepG2细胞的葡萄糖代谢、胰岛素抵抗和氧化应激具有改善作用。明确了羊栖菜水提物通过激活Nrf2/ARE信号通路改善氧化应激和胰岛素抵抗,治疗Ⅱ型糖尿病的潜力。The main symptoms of type 2 diabetes include insulin resistance,high blood sugar and insulin deficiency.Al-though some oral hypoglycemic drugs can be effective in improving insulin resistance,most of them,including biguanides,thiazolidinediones,and sulfonylureas,cause serious adverse reactions,which has led to an interest in naturally sourced drugs to treat type 2 diabetes.In this study,the mechanism of the improvement of oxidative stress and insulin resistance of HepG2 cells by water extract of Yangxiensis based on Nrf2/ARE signaling pathway was investigated.After treatment with different concentrations of water extract of Yangxiensis,superoxide dismutase and glutathione peroxidase significantly in-creased,and the levels of reactive oxygen species and lipid malondialdehyde oxide significantly reversed.Meanwhile,the levels of nuclear factor erythrocyte-related factor 2,Kelch-like epichlorohydrin-related protein and heme oxygenase-1 protein significantly increased in insulin-resistant HepG2 cells.And the gene expressions of nuclear factor E2 related fac-tor 2,heme oxygenase 1 and insulin receptor substrate 1 significantly increased,which further confirmed that the water ex-tract of Yangzi can improve glucose metabolism,insulin resistance and oxidative stress of HepG2 cells induced by high glu-cose resistance.This study identified the potential of the water extract of Herba japonica to improve oxidative stress and in-sulin resistance and treat type 2 diabetes by activating Nrf2/ARE signaling pathway.

关 键 词：羊栖菜水提物 Ⅱ型糖尿病 Nrf2/ARE信号通路 

分 类 号：Q257[生物学—细胞生物学]