山茱萸山药水提物对环磷酰胺诱导的免疫抑制小鼠免疫功能及其肠道微生物的影响  

Effects of CORNI FRUCTUS-DIOSCOREAE RHIZOMA Aqueous Extract on Immune Function and Intestinal Flora in Cyclophosphamide-Induced Immunosuppressed Mice

作　　者：杨菊花 李常学 郑璐瑶 张宇佳 田韦韦 唐碧华 余东梅 刘俐 赵军宁 华桦 YANG Juhua;LI Changxue;ZHENG Luyao;ZHANG Yujia;TIAN Weiwei;TANG Bihua;YU Dongmei;LIU Li;ZHAO Junning;HUA Hua(Sichuan Institute for Translational Chinese Medicine,Sichuan Academy of Chinese Medicine Sciences,Key Lab.:Biological Evaluation of TCM Quality of the State Administration of Traditional Chinese Medicine,Translational Chinese Medicine Key Laboratory of Sichuan Province,Sichuan Provincial Engineering Research Center of Formation Principle and Quality Evaluation of Genuine Medicinal Materials,Sichuan Engineering Technology Research Center of Genuine Regional Drug,Chengdu 610041;College of Food and Biological Engineering,Chengdu University,Chengdu 610106;School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137)

机构地区：[1]四川省中医药转化医学中心,国家中医药管理局中药质量生物学评价重点研究室,中医药转化医学四川省重点实验室,四川省道地药材形成原理与品质评价工程研究中心,四川省道地药材系统开发工程技术研究中心,成都610041 [2]成都大学食品与生物工程学院,成都610106 [3]成都中医药大学药学院,成都611137

出　　处：《中药药理与临床》2024年第11期34-40,共7页Pharmacology and Clinics of Chinese Materia Medica

基　　金：四川省中医药科技产业创新团队专项(编号:2022C009);科技服务平台建设(编号:A-2022N-51)。

摘　　要：目的:探究山茱萸山药水提物对环磷酰胺诱导的免疫抑制小鼠免疫功能及其肠道微生物的影响。方法:将小鼠随机分为正常对照组、模型对照组、左旋咪唑片0.08 g/kg组、山茱萸山药水提物0.43、0.86、1.73 g/kg组,除正常对照组外,造模小鼠于第1~3 d腹腔注射40 mg/kg环磷酰胺。第4 d灌胃相应药物,连续14 d。末次给药24 h后,检测小鼠脏器指数、脾脏NK细胞百分率、外周血T、B淋巴细胞亚群比例,分析各组小鼠肠道菌群变化。结果:与正常对照组比较,模型对照组小鼠的体质量、胸腺指数、脾脏指数、CD8+T、CD19、CD19/CD13明显降低,NK细胞百分率明显升高(P<0.05或P<0.01);与模型对照组相比,左旋咪唑片0.08 g/kg组NK淋巴细胞百分率显著降低(P<0.01),山茱萸山药水提物1.73 g/kg组外周血CD19/CD3的百分率明显升高(P<0.05)。与正常对照组相比,模型对照组肠道菌群α多样性Shannon明显降低(P<0.05),疣微菌门显著降低(P<0.01),厚壁菌门明显升高(P<0.05);与模型对照组比较,山茱萸山药水提物0.43 g/kg组肠道中疣微菌门显著升高(P<0.01),山茱萸山药水提物0.86 g/kg组厚壁菌门明显降低(P<0.05),左旋咪唑片0.08 g/kg组拟杆菌门显著升高(P<0.05);在属水平分类上,与模型对照组比较,左旋咪唑片0.08 g/kg组另枝菌属、杆菌属明显升高(P<0.05或P<0.01),山茱萸山药水提物0.86 g/kg组肠道中杆菌属明显升高、Lachnoclostridium明显降低(P<0.05或P<0.01),山茱萸山药水提物1.73 g/kg组Lachnoclostridium显著降低(P<0.01)。结论:山茱萸山药水提物在一定程度上可以改善环磷酰胺诱导的小鼠免疫功能异常和肠道菌群失调。Objective:To investigate the effects of CORNI FRUCTUS-DIOSCOREAE RHIZOMA aqueous extract on the immune function and intestinal flora in cyclophosphamide-induced immunosuppressed mice.Methods:The mice were randomly divided into a normal control group,a model group,a 0.08 g/kg levamisole tablets group,and 0.43,0.86,and 1.73 g/kg CORNI FRUCTUS-DIOSCOREAE RHIZOMA aqueous extract groups,with 10-11 mice in each group.Except for the normal control group,mice in the other groups were intraperitoneally injected with 40 mg/kg cyclophosphamide on the first three days.From the 4th day,the corresponding drugs were given by gavage for 14 consecutive days.After 24 hours of the last administration,the organ indexes,the percentage of NK cells in the spleen,the proportions of T and B lymphocyte subsets in peripheral blood of mice in each group were determined,and the changes in intestinal flora in mice of each group were analyzed.Results:Compared with those of the normal control group,the body weight,thymus index,spleen index,CD8~+T,CD19,and CD19/CD13 were significantly decreased in the model control group,while the percentage of NK cells was significantly increased(P<0.05 or P<0.01).Compared with those of the model control group,NK lymphocytes in the levamisole tablets group were significantly decreased(P<0.01),while the CD19/CD13 ratio was significantly increased after the intervention by 1.73 g/kg CORNI FRUCTUS-DIOSCOREAE RHIZOMA aqueous extract(P<0.05).Compared with the normal control group,the model control group showed a significantly decreased Shannon index(P<0.05),which reflected the alpha diversity of intestinal flora,significantly decreased Verrucomicrobia(P<0.01),and significantly increased Firmicutes(P<0.05).In comparison with the model control group,the 0.43 g/kg CORNI FRUCTUS-DIOSCOREAE RHIZOMA aqueous extract group saw significantly increased Verrucomicrobia(P<0.01),the 0.86 g/kg CORNI FRUCTUS-DIOSCOREAE RHIZOMA aqueous extract group witnessed significantly decreased Firmicutes(P<0.05),the 0.08 g/kg levamisole table

关 键 词：山茱萸山药水提物 环磷酰胺 免疫抑制 淋巴细胞亚群 肠道菌群 

分 类 号：R28[医药卫生—中药学]