电离辐射对体外血管内皮细胞早衰的影响  

The effect of ionizing radiation on premature death of vascular endothelial cells in vitro

作　　者：伊如罕 陆雪[1] 蔡恬静[1] 高玲[1] YI Ruhan;LU Xue;CAI Tianjing;GAO Ling(Key Laboratory of Radiological Protection and Nuclear Emergency,National Institute for Radiological Protection,Chinese Center for Disease Control and Prevention,Beijing 100088,China)

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,辐射防护与核应急中国疾病预防控制中心重点实验室,北京100088

出　　处：《癌变.畸变.突变》2025年第1期16-20,共5页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:探讨人微血管内皮细胞(HMEC-1)在电离辐射照射后是否发生早衰及其产生的机制。方法:以剂量率11.79 mGy/s的X射线照射HMEC-1细胞,设置为0(对照组)、2和4 Gy剂量组,分别照射0、2.83和5.65 min后继续培养24、48和72 h。采用CCK-8试剂盒检测电离辐射暴露后细胞活力的变化;β-半乳糖苷酶染色试剂盒检测辐照后细胞衰老的发生情况;利用吉姆萨染色观察辐照后细胞形态变化;Western blot法检测衰老标志蛋白P53和P21的表达水平。结果:与对照组相比,4 Gy剂量组照射后0 h,细胞活力显著下降(P<0.05);2和4 Gy X射线照射后24、48和72 h的细胞活力均显著下降(P<0.01);且2和4 Gy X射线照射后72 h的细胞β-半乳糖苷酶染色显著增加(P<0.05)。与未发生衰老细胞相比,发生早衰细胞的体积变大,随着照射剂量增加,细胞形态变化越明显;与对照组相比,照射组细胞衰老相关蛋白P53和P21的表达水平均显著升高(P<0.05)。结论:电离辐射可通过激活P53-P21通路引起HMEC-1细胞发生早衰。OBJECTIVE:To investigate whether premature aging occurs after irradiation of human microvascular endothelial cells(HMEC-1)with ionizing radiation and the mechanism of its generation.METHODS:HMEC-1 cells were irradiated with X-rays at a dose rate of 11.79 mGy/s for 0,2 and 4 Gy,for 0,2.83 and 5.65 min,respectively,and cultured for 24,48 and 72 h after irradiation.The CCK-8 kit was used to detect the changes in cell viability after exposure to ionizing radiation;the β-galactosidase staining kit was used to detect the onset of cellular senescence after irradiation;Giemsa staining was used to observe the changes in the morphology of irradiated cells;and the expression levels of the senescence marker proteins,P53 and P21,were detected using the Western blot method.RESULTS:Compared with the unirradiated group,cell viability decreased significantly at 0 h after irradiation in the 4 Gy dose group(P<0.05);cell viability decreased significantly at 24,48 and 72 h after irradiation in the 2 and 4 Gy X-ray irradiation groups(P<0.01);andβ-galactosidase staining of the cells at 72 h after irradiation in the 2 and 4 Gy X-rays irradiation groups increased significantly compared with the unirradiated group(P<0.05).And compared with the non-senescent cells,the morphology of the cells that underwent premature senescence became larger,and with the increase of irradiation dose,the more obvious the changes in cell morphology;compared with the non-irradiated group,the expression level of cellular senescence-related proteins P53 and P21 in the irradiated group was significantly elevated(P<0.05).CONCLUSION:Ionizing radiation can cause premature senescence of HMEC-1 cells by activating the P53-P21 pathway.

关 键 词：电离辐射 X射线 血管内皮细胞 早衰 

分 类 号：Q691.5[生物学—生物物理学]