基于植物形态和DNA分子技术鉴定党参属五种植物  

Identification of Five Species of the Codonopsis on Plant Morphological and DNA Molecules

作　　者：张志宇 刘付松 刘莎 张德林 王化东[3] 吴发明 ZHANG Zhiyu;LIU Fusong;LIU Sha;ZHANG Delin;WANG Huadong;WU Faming(Guizhou Medical Products Administration Inspection Centre,Guiyang,Guizhou 550081;College of Pharmacy,Zunyi Medical University,Zunyi,Guizhou 563000;College of Pharmacy,Sichuan College of Traditional Chinese Medicine,Mianyang,Sichuan 621000)

机构地区：[1]贵州省药品监督管理局检查中心,贵州贵阳550081 [2]遵义医科大学药学院,贵州遵义563000 [3]四川中医药高等专科学校药学院,四川绵阳621000

出　　处：《北方园艺》2025年第2期14-22,共9页Northern Horticulture

基　　金：国家自然科学基金资助项目(82060687,81760679);贵州省科技支撑计划资助项目(黔科合支撑[2021]一般248)。

摘　　要：以13个省市的106份党参种质资源为试材,采用植物形态和DNA分子鉴定相结合的方法,研究了106份党参种质资源间的亲缘关系,以期为植物形态特征和DNA分子技术鉴定党参种质资源提供参考依据。结果表明:党参、川党参、素花党参与管花党参、羊乳在植物形态上有较大的差异,而党参、川党参和素花党参之间差异较小,但均可以通过植物形态差异进行鉴别;利用ITS2序列和psbA-trnH序列可准确鉴别党参、川党参、素花党参与管花党参、羊乳,但对变种与原变种间的鉴定仍存在一定局限性。Taking 106 Codonopsis resources from 13provinces and cities as the test materials,the genetic relationship of 106 Codonopsis germplasm resources was studied by the combination of plant morphological characteristics and DNA molecular identification,in order to provide reference for identifying Codonopsis germplasm resources by DNA barcoding combined with plant morphological characteristics.The results showed that there were significant morphological differences among C.pilosula(Franch.)Nannf.,C.tangshen Oliv.,C.pilosula Nannf.var.modesta(Nannf.)L.T.Shen as well as C.tubulosa,C.lanceolata.Differences between C.pilosula(Franch.)Nannf.,C.tangshen Oliv.and C.pilosula Nannf.var.modesta(Nannf.)L.T.Shen were smaller,but all could be identified by plant morphological differences.ITS2and psbA-trnH sequences accurately identified C.pilosula(Franch.)Nannf.,C.tangshen Oliv.,C.pilosula Nannf.var.modesta(Nannf.)L.T.Shen as well as C.tubulosa,C.lanceolata.However,limitations were encountered in identifying variant species among the original variants.

关 键 词：党参 植物形态 DNA分子 ITS2序列 psbA-trnH序列 

分 类 号：S567.79[农业科学—中草药栽培]