灵芝木霉病病原菌分离鉴定及抗病种质筛选  

Isolation and Identification of Pathogen of Trichoderma Ganoderma and Screening of Resistant Germplasm

作　　者：贺黎铭 蔡凌 谢欣 刘林秋 余梦瑶[1,2] 罗霞 HE Liming;CAI Ling;XIE Xin;LIU Linqiu;YU Mengyao;LUO Xia(Sichuan Academy of Chinese Medicine Science,Chengdu,Sichuan 610041;Sichuan-Chongqing Joint Key Laboratory of Innovation of New Drugs of Traditional Chinese Medicine,Chengdu,Sichuan 610041)

机构地区：[1]四川省中医药科学院,四川成都610041 [2]中药新药创制川渝共建重点实验室,四川成都610041

出　　处：《北方园艺》2025年第2期59-66,共8页Northern Horticulture

基　　金：四川省省级科研院所基本科研业务费资助项目(2023JDKY0027);成都市科技资助项目(2024-YF05-02175-SN,2019-YF05-02432-SN);四川省科技计划资助项目(2021YFYZ0012);国家现代农业产业技术体系四川道地中药材创新团队资助项目(SCCXTD-2020-19);四川省“天府万人计划”资助项目(川万人第407号)。

摘　　要：以染病灵芝菌袋和子实体为试材,采用组织分离纯化法、ITS序列测序、平板对峙、菌袋筛选等方法,研究了灵芝木霉病病原菌的分类地位及抗病种质筛选,以期为木霉病的绿色防治提供参考依据。结果表明:4个木霉菌株被鉴定为木霉属的3个种,D14和JY-2为哈茨木霉(Trichoderma harzianum),JY-1为侧耳木霉(T.pleuroticola),Lv为长枝木霉(T.longibrachiatum)。4个木霉菌株在平板上和袋料中的侵染性强弱表现基本一致,从种类上侵染性强弱表现为侧耳木霉>哈茨木霉>长枝木霉。不同灵芝菌株与4个木霉菌株的抗性情况明显不同。该研究成功筛选获得1个抗木霉病的优质灵芝菌株ZL18,为高抗性优质灵芝新品种的选育和木霉病的有效防治奠定基础。Ganoderma lucidum bag and fruiting body were used as the experimental materials.The taxonomic status of pathogen of Trichoderma Ganoderma and the screening of disease-resistant strains were studied by using the tissue isolation and purification technology method,ITS sequencing and plate confrontation,bag screening,in order to provide reference for the green control of Trichoderma.The results showed that the four strains were identified as three species of Trichoderma,D14 and JY-2 as Trichoderma harzianum,JY-1 as T.pleuroticola,and Lv as T.longibrachiatum.The infectivity of the four Trichoderma strains on the agar plate and in the bag was basically the same,and the infectivity in terms of species was as follows,T.pleuroticola>T.harzianum>T.longibrachiatum.There were significant differences among G.lucidum strains in resistance to four Trichoderma strains.A high-quality G.lucidum strain ZL18 resistant to Trichoderma was screened,laying the foundation for the breeding of new high resistant and highquality G.lucidum variety and effective prevention and control of trichoderma ganoderma.

关 键 词：灵芝 木霉 抗性筛选 

分 类 号：S567.31[农业科学—中草药栽培]