铜暴露对黄条鰤胚胎和初孵仔鱼发育的毒性效应  

Effects of copper exposure on embryonic and yolk-sac larval development of Seriola aureovittata:Ecological and molecular toxicity

作　　者：靳智欣 徐永江[1,2] 崔爱君 姜燕 王滨[1,2] 刘新富 JIN Zhixin;XU Yongjiang;CUI Aijun;JIANG Yan;WANG Bin;LIU Xinfu(State Key Laboratory of Mariculture Biobreeding and Sustainable Goods,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;Qingdao Marine Science and Technology Center,Qingdao 266237,China)

机构地区：[1]中国水产科学研究院黄海水产研究所海水养殖生物育种与可持续产出全国重点实验室,山东青岛266071 [2]青岛海洋科技中心,山东青岛266237

出　　处：《中国水产科学》2024年第10期1186-1203,共18页Journal of Fishery Sciences of China

基　　金：国家海水鱼产业技术体系项目(CARS-47);山东省重点研发计划项目(2023TZXD050,2021SFGC0701);泰山产业领军人才工程、青岛市海洋科技创新专项(24-1-3-hygg-22-hy);中国水产科学研究院基本科研业务费项目(2023TD51,2024XT0701)。

摘　　要：为研究铜对黄条鰤(Seriola aureovittata)胚胎和初孵仔鱼发育的生理生态毒性效应,将胚胎和初孵仔鱼分别暴露于0(对照)、0.034、0.060、0.107、0.192、0.340、0.600、1.070mg/L的CuSO_(4)溶液中60h和5d,分析Cu暴露对胚胎发育、孵化率和初孵仔鱼的生长发育、存活、营养利用以及畸形率的影响,分别计算Cu对胚胎和初孵仔鱼的半致死质量浓度(LC_(50))、安全质量浓度(SC)。同时,利用转录组测序分析了卵黄囊仔鱼对Cu暴露胁迫的分子应答特性。结果显示:(1)Cu胁迫下黄条鰤出现胚胎发育畸形与孵化率低、初孵仔鱼畸形率高及卵黄囊利用速率快等现象。(2)胚胎阶段Cu^(2+)的48h-LC_(50)为0.08mg/L远低于初孵仔鱼阶段(0.60mg/L),Cu对黄条鰤初孵仔鱼的SC为0.034mg/L。(3)Cu暴露下,胚胎中差异表达基因(DEGs)主要富集在氧化磷酸化信号通路上,初孵仔鱼中DEGs主要富集在细胞周期以及光信号传导通路中。研究结果为黄条鰤早期发育阶段铜制剂的安全使用浓度以及水环境的生物监测提供理论依据。Copper is an important heavy metal and an essential trace element for aquatic organisms and is found at low concentrations in aquatic ecosystems.In aquaculture,copper sulfate is used as a therapeutic agent to reduce parasitic infections in cultured fish.However,exposure to heavy metals beyond a safe concentration range can cause a series of physiological and biochemical stresses in aquatic animals,including death.Fish are generally most sensitive to the effects of Cu~(2+)exposure during early life stages,especially during embryonic and larval development,which can result in reduced fertilization success,chromosomal abnormalities,DNA injury,increased incidence of yolk membrane ruptures,reduced hatching success and survival,altered time to hatching,embryonic and larval teratogenicity,inhibited growth,abnormal larval behaviors.Yellowtail kingfish(Seriola aureovittata)is a fast-growing marine species widely distributed in temperate and subtropical regions of the oceans.It has a large body size,superior flesh quality,high economic value,and can be cultured in sea cages,indoor recirculating aquaculture systems,aquaculture crafts,and other culture modes.Therefore,S.aureovittata is a promising candidate for open-ocean aquaculture in China.To investigate the effects of copper exposure on the early life stages of S.aureovittata,embryos and yolk sac larvae were exposed to copper concentrations of 0,0.034,0.060,0.107,0.192,0.340,0.600,and 1.070 mg/L for 60 h and 5 d under laboratory conditions in the present study.The half-lethal concentration(LC_(50))and safe concentration(SC)were determined for embryos and newly hatched larvae of S.aureovittata,respectively.In addition,differential gene expression patterns and related signaling pathways were investigated in embryos and yolk-sac larvae to elucidate possible molecular mechanisms.The results showed that:(1)Low hatching rate of embryos,high malformation rate of newly hatched larvae,and high utilization speed of yolk sacs during the early developmental stage of S.aureovittata u

关 键 词：黄条鰤 铜暴露 胚胎发育 仔鱼生长 急性毒性效应 转录组分析 

分 类 号：S917[农业科学—水产科学]