南海海绵共附生放线菌的抗菌活性筛选及菌株HMg33的鉴定  

Screening of antimicrobial sponge-associated actinomycetes from the South China Sea and identification of strain HMg33

作　　者：杨芳丽 李姿颖 徐欣雨 曹旭霞 周越 张光涛 YANG Fangli;LI Ziying;XU Xinyu;CAO Xuxia;ZHOU Yue;ZHANG Guangtao(School of Pharmacy,Binzhou Medical University,Yantai 264003,Shandong,P.R.China)

机构地区：[1]滨州医学院药学院,山东烟台264003

出　　处：《滨州医学院学报》2024年第6期440-445,451,共7页Journal of Binzhou Medical University

基　　金：滨州医学院科研启动基金(BY2022KYQD47);大学生创新创业训练计划(X202310440648)。

摘　　要：目的研究一种南海海绵共附生可培养放线菌的物种多样性及其抗菌活性,检测其中稀有放线菌菌株生物合成药物先导化合物的潜力。方法采用平板分离法分离南海海绵共附生放线菌,基于形态学排重和16S rDNA基因序列鉴定所得放线菌种属;采用琼脂块免提取法初步测试菌株的抗菌活性。应用antiSMASH软件预测放线菌最相似菌株的生物合成潜力。通过单菌株多化合物策略优化培养条件,高效液相色谱检测菌株发酵产物的指纹图谱。结果从该南海海绵样品中共获得链霉菌及稀有放线菌22株,其中68%菌株对供试病原菌展现出抗菌活性,尤其是稀有放线菌HMg33使用OSMAC策略发现粗提物可抑制耐甲氧西林金黄色葡萄球菌。结论南海海绵共附生放线菌具有物种多样性和广谱抗菌活性,菌株HMg33可作为药源微生物进行抗感染药物先导物的挖掘。Objective To investigate the species diversity and antibacterial activity of culturable actinomycetes associated with the South China Sea sponge,and to evaluate the potential of rare actinomycetes strains for biosynthesis of drug lead compounds.Methods Nice selective media were used for isolation of sponge-associated actinomycetes.Colony morphology observation and 16S rDNA gene sequencing technology were employed to identify the actinomycetes isolates.The agar block non-extraction method was used to test preliminarily the antibacterial activity of strains.antiSMASH software was used to predict the biosynthetic potential of the most similar strains of actinomycetes.One strain-many compounds(OSMAC)strategy was applied to optimize the culture conditions and HPLC was used to detect the fingerprint profiles of selected strain fermentation products.Results Among 22 strains of Streptomyces and rare actinomyces obtained from the South Sea sponge samples,68%of the strains showed antibacterial activity against the tested pathogens.Especially,the crude extract of rare actinomycete HMg33 showed inhibitory activity against clinical drug-resistant bacteria MRSA with the OSMAC strategy.Conclusion The actinomycetes associated with sponges in the South China Sea exhibit species diversity and broad-spectrum antibacterial activity.The strain HMg33 displays promise to be used as the microbial source for anti-infective drug leads discovery.

关 键 词：南海海绵 放线菌 抗菌活性 耐甲氧西林金黄色葡萄球菌 生物合成潜力 

分 类 号：R96[医药卫生—药理学]