木棉花不同部位UPLC指纹图谱建立及抗氧化谱效关系研究  

Establishment of UPLC Fingerprint of Different Parts of Gossampim Flos and Study on Spectrum Effect Relationship

作　　者：余欣彤 曹嵌 张兰兰 汪凯东 陈灶树 潘玲 张正 YU Xintong;CAO Qian;ZHANG Lanlan;WANG Kaidong;CHEN Zaoshu;PAN Ling;ZHANG Zheng(Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule,Guangdong Yi Fang Pharmaceutical Co.,Ltd.,Foshan 528244,China)

机构地区：[1]广东一方制药有限公司,广东省中药配方颗粒企业重点实验室,广东佛山528244

出　　处：《中国药学杂志》2024年第22期2132-2139,共8页Chinese Pharmaceutical Journal

基　　金：中华人民共和国工信部2022年产业技术基础公共服务平台项目资助(2022-230-221);佛山市南海区重点领域科技攻关专项资助(南科﹝2023﹞20号-18)。

摘　　要：目的比较木棉花不同部位水提取物抗氧化活性并建立超高效液相色谱(UPLC)指纹图谱,研究谱效关系,揭示其抗氧化活性质量标志物。方法以2,2-联氮-二(3-乙基-苯并噻唑啉-6-磺酸)二铵盐(ABTS)与1,1-二苯基-2-苦基肼基自由基(DPPH)自由基清除率为指标,采用UPLC法建立12批木棉花不同部位(花瓣、花萼、雄蕊)指纹图谱,进行相似度分析,结合灰色关联度分析(GRA)、斯皮尔曼(Spearman)分析、逐步回归分析、偏最小二乘回归(PLSR)分析对抗氧化谱效关系进行研究。结果12批木棉花不同部位水提取物指纹图谱共标定并指认8个色谱峰。芒果苷、槲皮苷、芦丁、牡荆苷等化合物为木棉花药材及其不同部位抗氧化活性的主要有效成分。结论木棉花药材及其不同部位抗氧化活性是多组分共同联合效应的结果,本研究可为木棉花药材及其不同部位抗氧化活性物质基础及质量控制提供参考。OBJECTIVE To determine the antioxidant activities of Gossampim Flos waterextracts,establish UPLC fingerprints and study the spectral effect relationship.METHODS Based on the scavenging rates of 2,2-diazo-di(3-ethyl-benzothiazolin-6-sulfonic acid)diamiammonium salt(ABTS)and 1,1-diphenyl-2-picrohydrazyl radical(DPPH),the fingerprint of different parts(petal,calyx and stamen)of 12 batches of Gossampim Flos were established by UPLC method,and similarity analysis were carried out.Grey correlation analysis(GRA),Spearman analysis,stepwise regression analysis and partial least squares regression(PLSR)analysis were used to study the relationship between antioxidant spectrum effect.RESULTS Eight chromatographic peaks were identified by fingerprint identification of water extracts from different parts of 12 batches of Gossampim Flos.Mangiferin,quercetin,rutin,vitexin and other compounds were the main active components of Gossampim Flos and its different parts for antioxidant activities.CONCLUSION The antioxidant activity of Gossampim Flos and its different parts are the result of the combined effect of multiple components.This study can provide a comprehensive reference for the basis and quality control of antioxidant active substances of Gossampim Flos and its different parts.

关 键 词：木棉花 抗氧化 指纹图谱 2 2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐 1 1-二苯基-2-苦基肼基自由基 谱效关系 

分 类 号：R282[医药卫生—中药学]