α/βhydrolase domain-containing protein 1 acts as a lysolipid lipase and is involved in lipid droplet formation  

作　　者：Ismael Torres-Romero Bertrand Légeret Marie Bertrand Damien Sorigue Alicia Damm Stéphan Cuiné Florian Veillet Carla Blot Sabine Brugière Yohann Couté Matthew G.Garneau Hari K.Kotapati Yi Xin Jian Xu Philip D.Bates Abdou R.Thiam Fred Beisson Yonghua Li-Beisson 

机构地区：[1]Aix Marseille Univ,CEA,CNRS,Institute of Bioscience and Biotechnology of Aix Marseille,BIAM,Saint-Paul-Lez-Durance 13108,France [2]Laboratoire de Physique de l’École Normale Supérieure,ENS,UniversitéPSL,CNRS,Sorbonne Université,Universitéde Paris Cité,Paris 75005,France [3]Univ.Grenoble Alpes,INSERM,CEA,UMR BioSantéU1292,CNRS,CEA,FR2048,Grenoble 38000,France [4]Institute of Biological Chemistry,Washington State University,Pullman 99164,USA [5]Single-Cell Center,CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,China [6]State Key Laboratory of Marine Resource Utilization in the South China Sea,Hainan University,Haikou 570228,China

出　　处：《National Science Review》2024年第12期144-154,共11页国家科学评论(英文版)

基　　金：supported by the European Union Regional Developing Fund(ERDF),the Région Provence Alpes Côte d’Azur,the French Ministry of Research and the French Alternative Energies and Atomic Energy Commission(CEA)by way of funding the HelioBiotec platform;supported by Fundamental Research Division(DRF)Impulsion LD power;I.T.R.is the recipient of an international PhD studentship from CEA(program Irtelis).A.R.T.is supported by ANR-21-CE11-0032-02-LIPRODYN.M.G.G.,H.K.K;P.D.B.are supported by the National Science Foundation(PGRP-IOS-1829365);The proteomic experiments were par tially suppor ted by Agence Nationale de la Recherche under projects ProFI(Proteomics French Infrastructure,ANR-10-INBS-08);GRAL(Grenoble Alliance for Integrated Structural&Cell Biology),a program from the Chemistry Biology Health(CBH)Graduate School of University Grenoble Alpes(ANR-17-EURE-0003).

摘　　要：Lipid droplets(LDs)are the major sites of lipid and energy homeostasis.However,few LD biogenesis proteins have been identified.Using model microalga Chlamydomonas,we show that ABHD1,anα/β-hydrolase domain-containing protein,is localized to the LD surface and stimulates LD formation through two actions:one enzymatic and one structural.The knockout mutants contained similar amounts of triacylglycerols(TAG)but their LDs showed a higher content of lyso-derivatives of betaine lipid diacylglyceryl-N,N,N-trimethylhomoserine(DGTS).Over-expression of ABHD1 increased LD abundance and boosted TAG content.Purified recombinant ABHD1 hydrolyzed lyso-DGTS,producing a free fatty acid and a glycery ltrimethy lhomoserine.In vitro droplet-embedded vesicles showed that ABHD1 promoted LD emergence.Taken together,these results identify ABHD1 as a new player in LD formation by its lipase activity on lyso-DGTS and by its distinct biophysical property.This study further suggests that lipases targeted to LDs and able to act on their polar lipid coat may be interesting tools to promote LD assembly in eukaryotic cells.

关 键 词：ALGAE betaine lipid α/βhydrolase lipid droplet lysolipid 

分 类 号：Q51[生物学—生物化学]